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GB/T 44482-2024: Chemicals - Tetrahymena thermophila multi-generational reproductive test
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| GB/T 44482-2024 | English | 359 |
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Chemicals - Tetrahymena thermophila multi-generational reproductive test
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Basic data
| Standard ID | GB/T 44482-2024 (GB/T44482-2024) |
| Description (Translated English) | Chemicals - Tetrahymena thermophila multi-generational reproductive test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | R04 |
| Classification of International Standard | 13.300 |
| Word Count Estimation | 18,123 |
| Date of Issue | 2024-09-29 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44482-2024: Chemicals - Tetrahymena thermophila multi-generational reproductive test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals - Tetrahymena thermophila multi-generational reproductive test
Multi-generation reproduction toxicity test of chemicals on Tetrahymena thermophila
ICS 13.300
CCS R 04
National Standard of the People's Republic of China
Released on 2024-09-29
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Test Principle ... 1
5 Test substance information ... 2
6 Reference substances ... 2
7 Test system ... 2
7.1 Instruments and Equipment 2
7.2 Materials and Reagents 2
7.3 Selection of test organisms 3
7.4 Test solution 3
7.5 Preparation before the test 3
7.6 Preliminary test 3
7.7 Formal test procedure 3
8 Limit test ... 4
9 Quality Control ... 5
10 Data and Reports ... 5
10.1 Calculation of inhibition rate 5
10.2 Calculation of ECx 5
10.3 Calculation of LOEC and NOEC ... 5
10.4 Test report 5
Appendix A (Informative) Guidelines for the Implementation of Laboratory Maintenance and Cultivation Methods of Tetrahymena thermophila ... 7
A.1 Overview 7
A.2 Commonly used culture media ... 7
A.3 Conservation of Tetrahymena thermophila 7
A.4 Cultivation of Tetrahymena thermophila ... 8
Appendix B (Informative) Reference table of different durations and generations of reproduction of Tetrahymena thermophila ... 9
Appendix C (Informative) Recommended 24-well plate setup for the Tetrahymena thermophila exposure test ... 10
Appendix D (Informative) Recommended 96-well plate setup for relative cell viability assay of Tetrahymena thermophila ... 11
References ... 12
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee on Hazardous Chemicals Management Standardization (SAC/TC 251).
This document was drafted by. China University of Geosciences (Wuhan), Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Microbiological Analysis and Detection Center
Center), China University of Mining and Technology, China Inspection and Testing (Tianjin) Co., Ltd., Ecological and Environmental Research Center of the Chinese Academy of Sciences, Ecological
South China Institute of Environmental Sciences, Ministry of Environment (Institute of Ecological and Environmental Emergency, Ministry of Ecology and Environment), Hubei Academy of Ecological and Environmental Sciences (Provincial Ecological and Environmental Science Research Institute)
Environmental Engineering Assessment Center), Linyi Inspection and Testing Center.
The main drafters of this document are. Liu Chunsheng, Zhang Yongkang, Mei Chengfang, Cheng Shiyang, Gao Liang, Xu Shengnan, Kong Ren, Li Haishan, Zhou Lili,
Qin Zhanfen, Dang Yao, Xiang Mingdeng, Yi Chuan, Liu Chaowu, Huo Jian, Zhang Yuling, Feng Lianna.
Multi-generation reproduction toxicity test of chemicals on Tetrahymena thermophila
1 Scope
This document describes the test principle, test substance information, reference substances, test system, and test results of the multi-generation toxicity test of Tetrahymena thermophila.
systems, limit testing, quality control, data and reporting.
This document applies to the multi-generation reproduction toxicity test of chemicals on Tetrahymena thermophila.
2 Normative references
This document has no normative references.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
tetrahymena thermophila density; TD
The number of Tetrahymena thermophila individuals contained in a unit volume [usually milliliters (mL)] of culture medium.
3.2
effect concentration; EC
The concentration of a test substance that results in a x% decrease in the density of Tetrahymena thermophila compared to the control group during a given test period.
Note 1.The English abbreviation for effect concentration is EC. ECx represents the concentration of the test substance when the inhibition rate is x%, such as EC50 represents the half-maximal effect concentration.
Note 2.The effect concentration is expressed as the amount of test substance contained in each unit volume of culture medium.
3.3
lowest observed effect concentration; LOEC
In a given assay period, the presence of a statistically significant effect (p < 0.05) on the density of Tetrahymena thermophila compared to the control group
Lowest test substance concentration.
Note. The p-value is a measure of the statistical significance of the observed results. When performing hypothesis testing, the significance level is usually set to
0.05, and p< 0.05 were considered to be statistically significant.
3.4
No observed effect concentration (NOEC)
No statistically significant effect (p ≥ 0.05) was observed on Tetrahymena thermophila density compared to the control group within a given assay period.
The highest test substance concentration.
Note. p ≥ 0.05 was considered to be statistically insignificant.
4 Test Principle
Thermophilic Tetrahymena can complete multiple generations of reproduction in a short test period. The addition of exogenous chemicals will affect its growth state and reproduction.
The hazard assessment of chemicals can be completed in a short time.
The density of Tetrahymena thermophila was measured in the culture medium, and the effect of exposure to the tested chemicals on the multi-generation reproduction of Tetrahymena thermophila was evaluated, and the
The effect concentration (ECx), the lowest observable effect concentration (LOEC) and the no observable effect concentration (NOEC) of the test substance.
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