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GB/T 41895-2022: Determination for DNA viruses of cell - MNP marker method
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Determination for DNA viruses of cell - MNP marker method
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GB/T 41895-2022
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Basic data | Standard ID | GB/T 41895-2022 (GB/T41895-2022) | | Description (Translated English) | Determination for DNA viruses of cell - MNP marker method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,140 | | Date of Issue | 2022-10-14 | | Date of Implementation | 2022-10-12 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 41895-2022: Determination for DNA viruses of cell - MNP marker method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination for DNA viruses of cell -- MNP marker method
ICS 07.080
CCSA40
National Standards of People's Republic of China
DNA virus assay in cells MNP labeling method
Published on 2022-10-12
2022-10-12 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
drafted.
Certain contents of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
This document was drafted by. Jianghan University, Wuhan Mingluo Biotechnology Co., Ltd., Hubei Provincial Center for Disease Control and Prevention, China Testing Technology
Research Institute of Biology, Beijing Technology and Business University, Science and Technology Development Center of the Ministry of Agriculture and Rural Affairs, Pony Testing Group Co., Ltd., Beijing Pony Medical
Scientific Laboratory Co., Ltd., Institute of Zoology, Chinese Academy of Sciences, Beijing Sambo Technology Co., Ltd.
The main drafters of this document. Peng Hai, Li Lun, Gao Lifen, Jiang Yongzhong, Zhou Lihua, Fang Bin, Ma Aijin, Zhou Junfei, Chen Hong, Fang Zhiwei, Liang Yong,
Li Tiantian, Chen Lihong, Xiao Huafeng, Wan Jing, Lu Yongming, Zhang Jing, Xiao Jinjin, Sun Zhaozeng, Liu Linlin, Cai Kun, Lu Jing, Zhou Kangping, Zhang Yating,
Zhang Ting, Yu Bo, Yu Xiao, Li Siting, Zhao Tongbiao, Hao Shuai.
DNA virus assay in cells MNP labeling method
1 Scope
This document describes reagents or materials for the determination of DNA (deoxyribonucleic acid) viruses in samples by MNP (polynucleotide polymorphism) labeling
materials, equipment, measurement procedures, quality control, calculation of results and presentation of results, anti-pollution measures and biosafety measures.
This document applies to adenovirus (Adenovirus, AdV), Epstein-Barrvirus (Epstein-Barrvirus, EBV), varicella-zoster disease
virus (Varicela-zostervirus, VZV), cytomegalovirus (Cytomegalovirus, CMV), herpes simplex virus type 1 (Herpes
The method specified in this document has a limit of detection (LOD) of 10 copies/reaction for a single virus.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB 19489 General Requirements for Laboratory Biosafety
GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
targetpathogen
One or more DNA viruses to be assayed.
3.2
internal control DNA
Synthetic DNA that has no homology with known biological genomes and has a definite copy number.
3.3
A polymorphism caused by multiple nucleotides in a nucleotide sequence not exceeding 300bp.
3.4
marker site marker
A nucleotide sequence on the genome of the target pathogen that specifically identifies the target pathogen, and the nucleotide sequence is related to the target pathogen
There is homology in the genome, and there is no homology with the genome of known organisms other than the target pathogen.
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