|
US$199.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 40458-2021: Specification of nucleic acid extraction used for high-throughput detection of pathogenic microorganisms
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 40458-2021 | English | 199 |
Add to Cart
|
3 days [Need to translate]
|
Specification of nucleic acid extraction used for high-throughput detection of pathogenic microorganisms
| Valid |
GB/T 40458-2021
|
PDF similar to GB/T 40458-2021
Basic data | Standard ID | GB/T 40458-2021 (GB/T40458-2021) | | Description (Translated English) | Specification of nucleic acid extraction used for high-throughput detection of pathogenic microorganisms | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 10,177 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40458-2021: Specification of nucleic acid extraction used for high-throughput detection of pathogenic microorganisms
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Specification of nucleic acid extraction used for high-throughput detection of pathogenic microorganisms
ICS 66.020.01
CCSB16
National Standards of People's Republic of China
For high-throughput detection of pathogenic microorganisms
Nucleic acid extraction technical specifications
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The publishing agency of this article does not bear the responsibility for identifying patents.
This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271).
This document was drafted by. China Academy of Inspection and Quarantine Science, Zhongshan Customs Technology Center.
The main drafters of this document. Jiang Fan, Zhang Yongjiang, Chen Jian, Yue Qiaoyun, Qiu Deyi.
For high-throughput detection of pathogenic microorganisms
Nucleic acid extraction technical specifications
1 Scope
This document specifies the nucleic acid extraction requirements for high-throughput detection of pathogenic microorganisms using second-generation sequencing technology, and describes the corresponding
method of extraction.
This document is applicable to the nucleic acid extraction methods of phytopathogenic bacteria, fungi, viruses, and vector-borne bacteria of plants and their products.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB 19489 Laboratory Biosafety General Requirements
SN/T 2752.4 Self-protection norms for health and quarantine personnel Part 4.Laboratory personnel
SN/T 2776 Management requirements for medical vector monitoring laboratories at frontier ports
SN/T 4278-2015 Operational Regulations for DNA Barcode Identification of Medical Vector Insects at Frontier Ports
SN/T 4625-2016 DNA barcode screening and quality requirements
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Abbreviations
The following abbreviations apply to this document.
DNA. Deoxyribonucleic Acid (Deoxyribonucleic Acid)
OD. Optical Density (OpticalDensity)
PCR. Polymerase Chain Reaction (PolymeraseChain Reaction)
RNA. Ribonucleic Acid (Ribonucleic Acid)
5 General requirements
Nucleic acid extraction mainly includes two major steps. lysis and purification of biological samples. Lysis is to free the nucleic acid in the sample in the lysis system
In the process, purification is to completely separate nucleic acid from other components in the lysis system, such as proteins, polysaccharides, lipids and other tissues or cell components.
The process of leaving.
Nucleic acid extraction should ensure the integrity of the primary structure of nucleic acid. Exclude other molecules, such as proteins, polysaccharides, lipids, organic solvents, etc.
To ensure the smooth progress of downstream high-throughput detection tests.
During the test, the external environment (such as dust, aerosol, RNase, etc.) should be prevented from contaminating the nucleic acid extraction, as well as the pathogen itself and the test.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 40458-2021_English be delivered?Answer: Upon your order, we will start to translate GB/T 40458-2021_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 40458-2021_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 40458-2021_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|