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GB/T 40453-2021: Detection and identification of Phyllosticta citricarpa(McAlpine) Aa
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Detection and identification of Phyllosticta citricarpa(McAlpine) Aa
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GB/T 40453-2021
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Basic data | Standard ID | GB/T 40453-2021 (GB/T40453-2021) | | Description (Translated English) | Detection and identification of Phyllosticta citricarpa(McAlpine) Aa | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 18,187 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40453-2021: Detection and identification of Phyllosticta citricarpa(McAlpine) Aa---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phyllosticta citricarpa(McAlpine) Aa
ICS 65.020.01
CCSB16
National Standards of People's Republic of China
Quarantine and identification method of citrus black spot pathogen
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with GB/T
1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was prepared by the National Plant Quarantine Standardization Technical Committee (SAC/TC
271) Propose and focus.
This document was drafted by. Fuzhou Customs of the People's Republic of China, Guangzhou Customs of the People's Republic of China, Zhejiang University, China Forestry Research Institute
Research Institute North China Forestry Experimental Center, Fujian Academy of Agricultural Sciences Institute of Agricultural Biological Resources, Hunan Institute of Plant Protection.
The main drafters of this document. Li Min, Wang Weifang, Li Hongye, Wang Xinghong, Li Xinfang, Zhou Xianzhi, Yu Yun, Zhang Zhuo, Shen Jianguo, Chen Xihong.
Quarantine and identification method of citrus black spot pathogen
1 Scope
This document specifies the quarantine and identification methods for citrus black spot pathogen.
This document is applicable to the isolation, detection and identification of citrus black spot pathogen in the seedlings and fruits of the citrus genus and its hybrids.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
SN/T
2455 Inspection and Quarantine Regulations for Entry and Exit Fruits
SN/T
4648 quarantine and identification method of citrus black spot pathogen
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Classification information
Scientific name. Phyllosticta
citricarpa
(McAlpine)
Aa,
Synonym. Guignardia
citricarpa
Kiely,
Phoma
citricarpa
McAlpine,
Phyllostictina
citricarpa
(McAlpine)
Petr.k,
Taxonomic status. Fungi, Ascomycota, Pezizomycotina, Do-
thideomycetes), Botryosphaeriales, Phylostictaceae, Phyllostic-
ta).
See Appendix A for the host range, geographic distribution, transmission mode, damage symptoms and morphological characteristics of citrus black spot pathogen.
5 Principles of the method
According to the symptoms of citrus black spot on the host plant, the suspected samples were isolated and cultured according to the morphological characteristics of the pathogen.
Signs and real-time fluorescent PCR specific reactions for identification.
6 Instruments and utensils
6.1 Apparatus
Stereo microscope, biological microscope, electronic balance (sensitivity 0.1
g), incubator, centrifuge, ultra-clean workbench, autoclave, water bath
Pot, vortex oscillator, ice maker, refrigerator, real-time fluorescent PCR machine.
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