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GB/T 40455-2021: Detection and identification of Blueberry shock virus
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Detection and identification of Blueberry shock virus
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GB/T 40455-2021
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Basic data | Standard ID | GB/T 40455-2021 (GB/T40455-2021) | | Description (Translated English) | Detection and identification of Blueberry shock virus�� | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 18,163 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40455-2021: Detection and identification of Blueberry shock virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Blueberry shock virus
ICS 65.020.01
CCSB16
National Standards of People's Republic of China
Quarantine and identification method of blueberry shock virus
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271).
This document was drafted by. Fuzhou Customs of the People's Republic of China, Fruit Research Institute of Fujian Academy of Agricultural Sciences, China Inspection and Quarantine Research Institute
Research Institute, Fujian Agriculture and Forestry University, Xiamen Customs of the People’s Republic of China.
The main drafters of this document. Shen Jianguo, Xie Lixue, Zhang Yongjiang, Cai Wei, Chen Xihong, Li Tao, Gao Fangluan, Li Min, Liao Furong.
Quarantine and identification method of blueberry shock virus
1 Scope
This document specifies the quarantine and identification method for Blueberryshockvirus.
This document is applicable to the quarantine and identification of blueberries that may carry blueberry shock virus.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 2122 Quarantine sampling method for entry and exit plants and plant products
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Classification information of blueberry shock virus
Chinese name. blueberry shock virus.
Scientific name. Blueberryshockvirus, abbreviation. BlShV.
Taxonomic status. Bromoviridae, Ilarvirus.
For the host range, disease symptoms, distribution area, transmission route, corpuscle morphology, genome and other information of blueberry shock virus, please refer to the attachment
Record A.
5 Principles of the method
The serological and molecular biological characteristics of blueberry shock virus are the main basis for formulating this quarantine identification method. According to BlShV and antibodies
The specific reaction between the samples is tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunochromatographic test strips;
Perform nested RT-PCR, immune capture nested RT-PCR, and real-time fluorescent RT-PCR detection according to the characteristics of the BlShV genome; through these methods
Effective combination of the method to determine whether the sample carries BlShV.
6 Instruments, utensils and reagents
6.1 Apparatus
High-speed refrigerated centrifuge, autoclave, electronic balance (sensitivity 0.001g), microplate reader, PCR instrument, real-time fluorescent PCR instrument, horizontal electric
Swimming system, conventional refrigerator, ultra-low temperature refrigerator, gel imaging analysis system, pH meter, constant temperature water bath, etc.
6.2 Utensils
Mortar, enzyme-linked plate, centrifuge tube, PCR reaction tube, adjustable pipette of various volumes (2.5μL, 10μL, 20μL, 100μL,.200μL,
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