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GB/T 40447-2021: Detection and identification of Rathayibacter rathayi(Smith)Zgurskaya et al.
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Detection and identification of Rathayibacter rathayi(Smith)Zgurskaya et al.
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GB/T 40447-2021
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Basic data | Standard ID | GB/T 40447-2021 (GB/T40447-2021) | | Description (Translated English) | Detection and identification of Rathayibacter rathayi(Smith)Zgurskaya et al. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,139 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40447-2021: Detection and identification of Rathayibacter rathayi(Smith)Zgurskaya et al.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Rathayibacter rathayi(Smith)Zgurskaya et al.
ICS 65.020.01
CCSB16
National Standards of People's Republic of China
Quarantine and identification method of Dactylis honey head pathogen
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271).
Drafting organizations of this document. Zhejiang Institute of Inspection and Quarantine Science and Technology, Hangzhou Customs of the People's Republic of China, Zhejiang University, China Metrology
University.
The main drafters of this document. Wu Zhiyi, Li Ke, Ren Yan, Huang Lingzhe, Li Bin, Zhang Pengjun, Tian Hongwei, Cheng Fan, Fang Wenyuan, Wu Ying, Chen Pengcheng.
Quarantine and identification method of Dactylis honey head pathogen
1 Scope
This document describes the quarantine and identification methods such as symptom observation, isolation and culture, and molecular biology of Dactylis vulgaris.
This document is applicable to the quarantine and identification of orchard grass, rye, ryegrass, wheat and other seeds and plants.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
SN/T 2122 Quarantine sampling method for entry and exit plants and plant products
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Basic information of germs
Chinese name. Dactylis honey head
Scientific name. Rathayibacterrathayi (Smith1913) Zgurskayaetal..1993
Synonyms. Clavibacterrathayi (Smith1913) Davisetal. 1984; Pseudobacterium rathayi (Smith1913)
Krasil'nikov1949; Agrobacteriumrathayi (Smith1913) Savulescu1947; Corynebacteriumrathayi
(Smith1913)Dowson1942;Erwiniarathayi (Smith1913)Grametal.1929;Phytomonasrathayi
(Smith1913)Bergeyetal.1923;Bacteriumrathayi(Smith1913)Aujeszky1914;Aplanobacter
rathayiSmith1913.
Taxonomic status. Bacteria, Actinobacteria, Actinobacteria, Actinobacteria
Actinomycetales, Microbacteriaceae, Rathayibacter.
See Appendix A for other basic information about Dactylis honey ear pathogen.
5 Principles of the method
According to the damage symptoms, culture characteristics, and physiological and biochemical characteristics of Dactylis vulgaris, the pathogen is isolated, cultured and tested for pathogenicity.
According to the specific DNA sequence of Dactylis vulgaris, molecular biology detection was carried out.
6 Equipment
Ultra-clean workbench, autoclave, microscope, incubator, electronic balance, centrifuge, refrigerator, real-time fluorescent PCR instrument, BIOLOG micro
Biometric identification system, etc.
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