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GB/T 39512-2020: Principle for phosphorylation labeled nucleic acid testing
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 39512-2020 | English | 189 |
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Principle for phosphorylation labeled nucleic acid testing
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GB/T 39512-2020
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Basic data | Standard ID | GB/T 39512-2020 (GB/T39512-2020) | | Description (Translated English) | Principle for phosphorylation labeled nucleic acid testing | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,137 | | Date of Issue | 2020-11-19 | | Date of Implementation | 2021-06-01 | | Quoted Standard | GB/T 6682; GB/T 30988; GB/T 30989; GB/T 34797 | | Regulation (derived from) | National Standard Announcement No. 26 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the terms and definitions, abbreviations, general requirements, determination methods, sample storage and reporting for phosphorylated labeled nucleic acid detection. This standard applies to the detection of phosphorylated-labeled nucleic acids used in the construction of DNA encoding compound libraries. | GB/T 39512-2020: Principle for phosphorylation labeled nucleic acid testing---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Principle for phosphorylation labeled nucleic acid testing
ICS 07.080
A40
National Standards of People's Republic of China
General rules for the detection of phosphorylated nucleic acid
2020-11-19 released
2021-06-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this standard. Institute of Biology, China Academy of Testing Technology, General Biosystems (Anhui) Co., Ltd., Sichuan Yazhongji
Yin Technology Co., Ltd., Shenzhen Institute of Metrology and Quality Inspection, China Academy of Testing Technology, Chengdu Pioneer Pharmaceutical Development Co., Ltd.
The company, Suzhou Jinweizhi Biotechnology Co., Ltd., West China Hospital of Sichuan University, Sichuan Agricultural University, Hebei Medical University.
The main drafters of this standard. Zhou Lihua, Yong Jingui, Korea Quan, Liu Zongwen, Yang Jiebin, Pan Hong, Li Huaiping, Zhang Yi, Ma Lixia, Yang Guowu,
Liu Qian, Jiang Zijing, Yang Li, Hao Junzheng, Zheng Yanyan, Lu Pin.
General rules for the detection of phosphorylated nucleic acid
1 Scope
This standard specifies the terms and definitions, abbreviations, general requirements, determination methods, sample storage and reporting for phosphorylated labeled nucleic acid detection.
This standard applies to the detection of phosphorylated-labeled nucleic acids used in the construction of DNA encoding compound libraries.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document.
For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 30988 Extraction, Purification and Testing Methods of Polyphenol Plant Genomic DNA
GB/T 30989 Technical Regulations for High-throughput Gene Sequencing
GB/T 34797 Quality and technical requirements for nucleic acid primer probes
3 Terms and definitions
The following terms and definitions defined in GB/T 34797 apply to this document.
3.1
Phosphorylated labeled nucleic acid
A single-stranded DNA with a length ranging from 9nt to 17nt is marked with a phosphate group at the 5'end.
3.2
DNA coding compounds
With 5'end labeled phosphate group, 3'end with 2~6 base sticky ends, formed by annealing two single-stranded DNAs of 9nt~17nt
Double stranded.
3.3
annealing
Two single-stranded DNAs are combined by heating denaturation and cooling renaturation according to the principle of complementary base pairing.
3.4
Complementary strand
The two single strands of a double-stranded nucleotide chain formed by base complementary pairing.
4 Abbreviations
The following abbreviations apply to this document.
DIEA. N,N-Diisopropylethylamine
DNA. deoxyribonucleic acid
EDTA. ethylenediaminetetraacetic acid
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