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GB/T 36848-2018: Detection and identification of Avocado sunblotch viroid
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| GB/T 36848-2018 | English | 199 |
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Detection and identification of Avocado sunblotch viroid
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GB/T 36848-2018
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Basic data | Standard ID | GB/T 36848-2018 (GB/T36848-2018) | | Description (Translated English) | Detection and identification of Avocado sunblotch viroid | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 10,125 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36848-2018: Detection and identification of Avocado sunblotch viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Avocado sunblotch viroid
ICS 65.020.01
B16
National Standards of People's Republic of China
Avocado spot plaque virus quarantine and identification method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. China Academy of Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people
Heguo Shandong Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Zhang Yongjiang, Zheng Yi, Zhang Jingyu, Xin Yanyan, Xiang Jun, Lu Xiaoyu.
Avocado spot plaque virus quarantine and identification method
1 Scope
This standard specifies avocado plaque virus reverse transcriptase polymerase chain reaction (RT-PCR) and reciprocating bidirectional polyacrylamide gel electrophoresis
Quarantine identification method.
This standard applies to the quarantine and identification of plants and their fruits that may carry avocado plaque.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Basic information
Chinese name. Avocado plaque virus
Scientific name. Avocadosunblotchviroid
Abbreviation. ASBVd
Classification status. Avsunviroidae Avsunviroid.
See Appendix A for additional information on avocado plaques.
4 Principle of the method
Preliminary determination based on the symptoms of ASBVd on host plants; based on its natural state of RNA and different structural molecules after denaturation
Reciprocating two-way polyacrylamide gel electrophoresis (Return-Polyacrylamide) in the difference of mobility in polyacrylamide gel electrophoresis
GelElectrophoresis, R-PAGE); RT-PCR detection based on genomic characteristics, results by electrophoresis band size
determination.
5 Equipment and facilities, appliances and reagents
5.1 Equipment and facilities
Balance (sensitivity 0.001g), high-speed refrigerated centrifuge, pH meter, PCR instrument, electrophoresis instrument, electrophoresis tank, gel imaging system, isolation and quarantine temperature
Room, constant temperature water bath, low temperature refrigerator, etc.
Micropipettes (2 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL), mortar, centrifuge tubes (1.5 mL, 2.0 mL), pots,
Disinfect the soil and so on.
5.2 RT-PCR detection reagent
Unless otherwise specified, all reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. See Appendix B for details.
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