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GB/T 36843-2018: Detection and identification of Candidatus Phytoplasma pyri
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36843-2018 | English | 359 |
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Detection and identification of Candidatus Phytoplasma pyri
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GB/T 36843-2018
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Basic data | Standard ID | GB/T 36843-2018 (GB/T36843-2018) | | Description (Translated English) | Detection and identification of Candidatus Phytoplasma pyri | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 18,127 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Regulation (derived from) | National Standard Announcement No. 11 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36843-2018: Detection and identification of Candidatus Phytoplasma pyri---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Candidatus Phytoplasma pyri
ICS 65.020.01
B16
National Standards of People's Republic of China
Pear degeneration phytoplasma quarantine identification method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fujian Entry and Exit Inspection of the People's Republic of China
Epidemic situation.
The main drafters of this standard. Wang Youfu, Li Xin, Yu Wentao, Liu Wei, Feng Wei, Yu Shuang, Wang Xiufen, Zhang Wei, Heidol, Liu Huiqiu,
Hu Qiang, Cao Dongmei.
Pear degeneration phytoplasma quarantine identification method
1 Scope
This standard specifies the quarantine and identification methods for the pear-degraded phytoplasma Candidatus Phytoplasmapyri.
This standard is applicable to the quarantine and identification of plants such as Rosaceae and Oleaceae and their reproductive materials and propagating mediators.
2 pear decay phytoplasma basic information
Chinese name. pear decay phytoplasma
Scientific name. CandidatusPhytoplasmapyriSeemüler
English name. peardecline; Parrysdisease; peachyelowleafrol; pearleafcurl; penarmoria
Classification status. Bacteria, Firmicutes, Molicutes, non-sterols
(Acholeplasmatales), Acholeplasmataceae, Phytoplasma, 16SrX-C
Prototype group.
See Appendix A for additional information.
3 Principle of the method
Pear decay phytoplasma symptoms and gene characteristics as the basis for the identification method of this standard.
4 instruments and main reagents
4.1 Instrumentation
Qualitative PCR instrument, quantitative PCR instrument, ultra-clean workbench, autoclave, high-speed refrigerated centrifuge, bench-top refrigerated centrifuge, pure water meter,
Bench-top small centrifuges, refrigerators, vortex shakers, micro-injectors, electrophoresis systems, water baths, gel imaging systems, etc.
4.2 Primary reagents
Only analytically pure reagents and deionized water were used in the analysis unless otherwise stated. The PCR reaction system uses double distilled water. DNA extraction test
The preparation can be prepared by referring to Appendix B. Other reagents, such as various solutions, buffers and culture media, are specific to the test methods, according to Appendix C, Appendix D.
And Appendix E requires preparation. Commercial reagents are described in their instructions for use.
Plant DNA extraction kit, PCR premix (PCRPremix), ultrapure water, DNA molecular marker (DNA Marker) and
DNA extraction reagents, etc.
Nucleic acid extraction slurry (100 mL). dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O), 2.17 g; potassium dihydrogen phosphate
(KH2PO4), 0.41 g; sucrose, 10 g; bovine serum albumin (BSA), 0.15 g; polyvinylpyrrolidone (PVP-10), 2 g. pH7.6
High temperature sterilization, stored at 4 ° C.
DNA extract. Tris-HCl, 100 mmol/L; EDTA, 100 mmol/L; NaCl, 250 mmol/L; pH adjusted to 8.0.
50 x TAE. 2.0 mmol/LTris, 1.0 mmol/L sodium acetate, 50 mmol/LEDTA, pH 8.0.
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