Search result: GB/T 35867-2018
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Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector
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GB/T 35867-2018
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| Standard ID | GB/T 35867-2018 (GB/T35867-2018) | | Description (Translated English) | Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.040 | | Word Count Estimation | 14,175 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine, China National Standardization Administration |
GB/T 35867-2018
Inspection of grain and oils - Determination of phospholipids content in lecithins - High performance liquid chromatography using a light-scattering detector
ICS 67.040
X04
National Standards of People's Republic of China
Determination of lecithin phospholipid content of grain and oil testing
High performance liquid chromatography with evaporative light scattering detection
(ISO 11701..2009, Vegetablefats andoils-Determination of phospholipids
contentinlecithinsbyHPLCusingalight-scatteringdetector, IDT)
2018-02-06 released
2018-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
1 range 1
2 Normative references 1
3 Terms and definitions 1
4 principle 1
5 Reagents and materials 1
6 instruments and equipment 2
7 sample stick 2
Preparation of test samples 2
9 Analysis Step 3
10 Results Calculation and Presentation 4
11 method precision 4
12 Test Report 4
Appendix A (informative) HPLC Figure 5
Appendix B (informative) joint laboratory test results 6
Reference 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard uses the translation method identical with ISO 11701..2009 "Edible vegetable oil HPLC Determination of Evaporative Light Scattering Detection
Phospholipids in the content of phospholipids. "
The documents of our country that are consistent with the corresponding international documents that are normative references in this standard are as follows.
Preparation of animal and vegetable fat samples (ISO 661..2003, IDT).
For ease of use, this standard made the following editorial changes.
--- Change the standard name (in order to facilitate the existing standard series).
This standard proposed by the State Food Administration.
This standard by the National Cereals and Oils Standardization Technical Committee (SAC/TC270) centralized.
This standard was drafted. Wuhan Food Cosmetics Inspection Institute, Wuhan Product Quality Supervision, Inspection, Wuhan University of Light Industry.
The main drafters of this standard. Li Jun, Jiang Xiaoming, Yang Yong, Lu Yupeng, Duan Jiankun, Hu Xiaojing, Fang Huiwen, He Dongping, Zhang Shihong, Zhou Yujuan,
Wang Fangfang, Wang Shu.
Determination of lecithin phospholipid content of grain and oil testing
High performance liquid chromatography with evaporative light scattering detection
1 Scope
This standard specifies the use of high performance liquid chromatography - evaporative light scattering detection quantitative determination of phospholipid content.
This standard applies to the crude oil lecithin, oil-free lecithin and lecithin extracted from plant oils.
As lysophosphatidylethanolamine, lysophosphatidylinositol, lysophosphatidic acid separation is not good, this standard does not apply to animal lecithin,
Ruminant lecithin and enzymatic lecithin.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
ISO 661 Animal and vegetable fats sample preparation (Animalandvegetablefatsandoils-Preparationoftestsam-
ple
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
One-component phospholipid contentofanindividualphospholipid
The N-acyl-phosphatidylethanolamine (N-acyl-PE), phosphatidylcholine (PC), phosphatidylethanol
Amine (PE), phosphatidylinositol (PI), phosphatidic acid (PA), lysophosphatidylcholine (LPC) mass fraction.
4 principle
Each phospholipid fraction was separated by a high performance liquid chromatograph equipped with a diol column, detected by an evaporative light scattering detector, and compared with a standard substance
Quantitative.
5 Reagents and materials
Warning - should strictly follow the rules for the use of toxic substances. Take effective measures to ensure the safety of organizations and individuals.
Unless otherwise specified, only analytical reagents are used.
5.1 water. a water.
5.2 n-Hexane. Chromatography pure.
Isopropyl alcohol. Chromatography pure.
Acetic acid. mass fraction ≥ 99.8%.
5.5 Triethylamine.
5.6 Mixed solvent. mixed solvent consists of 80 mL n-hexane (5.2) and 20 mL isopropanol (5.3) (n-hexane volume fraction
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