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GB/T 35542-2017: Taq DNA polymerase
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35542-2017 | English | 279 |
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Taq DNA polymerase
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GB/T 35542-2017
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PDF similar to GB/T 35542-2017
Standard similar to GB/T 35542-2017 GB/T 41907 GB/T 35540 GB/T 35539 Basic data | Standard ID | GB/T 35542-2017 (GB/T35542-2017) | | Description (Translated English) | Taq DNA polymerase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,172 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Regulation (derived from) | National Standards Bulletin 2017 No. 32 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35542-2017: Taq DNA polymerase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Taq DNA polymerase
ICS 07.080
C27
National Standards of People's Republic of China
Taq DNA polymerase
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 technical requirements
5 test methods 1
6 packaging, transportation and storage 2
7 shelf life 2
Appendix A (Normative) TaqDNA polymerase enzyme activity test 3
Appendix B (Normative) Exonuclease assay 6
Appendix C (Normative) Endonuclease assay 8
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized.
This standard drafting unit. China Agricultural University, Xiamen Zhi Shan Biotechnology Co., Ltd., Fujian Hua Can Pharmaceutical Co., Ltd., Fujian South
Health Technology Co., Ltd., Xiamen University, Hua Can Nan Sheng (Xiamen) Biotechnology Co., Ltd., Beijing University of Chemical Technology, Beaver Nano Technology (Suzhou)
Limited, Angel Yeast Co., Ltd., Fudan University, Shandong University, Fujian Agriculture and Forestry University, Suzhou University, Shanghai 100 赛 Biotechnology Co., Ltd.
Company, Shanghai Boshi Biological Technology Co., Ltd.
The main drafters of this standard. Songna Jie, Huangfa Can, Zheng Dengzhong, He Changhua, Zhan Xuexiong, Li Quanhong, Li Qing Court, Zhao Jing, Chen Jinchun,
Chen Xiulan, Yao Juan, Zhang Xiying, Liu Bin, Zhong Jiang, Huang Fa Xi, Ren Hui, Xing Zhigang, Zhu Li, Teresa, Liu Pei, Zhang Lili.
Introduction
Taq DNA polymerase is a thermostable enzyme derived from recombinant E. coli and contains all of the clones that are cloned from Thermus species
Long TaqDNA polymerase gene, molecular weight of 85ku. The temperature of 55 ℃ ~ 110 ℃ catalytic DNA replication, 74 ℃ ~ 75 ℃
The optimum temperature. TaqDNA polymerase to develop national standards to promote the industrialization of such tools, enzymes, TaqDNA polymerase
The production and use is of great significance.
Taq DNA polymerase
1 Scope
This standard specifies the technical requirements TaqDNA polymerase, test methods, packaging, transportation, storage and shelf life.
This standard applies to TaqDNA polymerase extracted from recombinant E. coli.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Taq DNA polymerase
A thermostable enzyme with a molecular mass of 85 ku derived from recombinant E. coli and containing the full length cloned from Thermus species
TaqDNA polymerase gene. The temperature of 55 ℃ ~ 110 ℃ catalytic DNA replication, 74 ℃ ~ 75 ℃ for the optimum temperature.
3.2
Taq DNA polymerase activity unit
Using a synthetic hairpin-type oligonucleotide sequence as a template/primer, 1.17 nmol of deoxynucleotides were polymerized into
The amount of enzyme required in double stranded DNA is 1 viable unit (U).
4 technical requirements
4.1 product appearance
Clear transparent liquid, no sediment.
4.2 enzyme activity
≥5000U/mL.
4.3 Impurities
It should not contain exonucleases and endonucleases.
5 test methods
5.1 appearance
Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.
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