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GB/T 35539-2017: Restriction endonuclease BamH Ⅰ
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35539-2017 | English | 279 |
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Restriction endonuclease BamH Ⅰ
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GB/T 35539-2017
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Standard similar to GB/T 35539-2017 GB/T 41907 GB/T 35541 GB/T 35540 Basic data | Standard ID | GB/T 35539-2017 (GB/T35539-2017) | | Description (Translated English) | Restriction endonuclease BamH �� | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,169 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Regulation (derived from) | National Standards Bulletin 2017 No. 32 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35539-2017: Restriction endonuclease BamH Ⅰ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Restriction endonuclease BamHⅠ
ICS 07.080
C27
National Standards of People's Republic of China
Restriction endonuclease BamHI
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 technical requirements
5 test methods 1
6 packaging, transportation and storage 2
7 shelf life 2
Appendix A (Normative) Restriction endonuclease BamH Ⅰ enzyme activity test 3
Appendix B (Normative) Other endonuclease detection 6
Appendix C (Normative) Exonuclease assay 7
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized.
This standard drafting unit. Beijing University of Chemical Technology, Fujian Nansheng Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., China Metrology Institute, Hill
East University, Sanming City Quality and Technical Supervision Association, Angel Yeast Co., Ltd., Fujian Agriculture and Forestry University.
The main drafters of this standard. Chen Jinchun, Chen Gang, Wang Liren, Huangfa Can, Quan Can, Zheng Dengzhong, Zhan Xuexiong, Zhuang Liqiong, Zheng Qiao Rong, Huang Fa,
Zhao Jing, Chen Xiulan, Yao Juan, Liu Bin.
Introduction
BamH I is a restriction endonuclease that specifically recognizes the G ↓ GATCC base sequence in the DNA strand and produces 5 '
End. Formulation of BamH I national standards, to promote the industrialization of such tools, enzymes, for the production and use of BamH I have important
significance.
Restriction endonuclease BamHI
1 Scope
This standard specifies the technical requirements of restriction endonuclease BamH Ⅰ, test methods, packaging, transport, storage and shelf life.
This standard applies to bacterial BacilusamyloliquefaciensH fermentation preparation or genetically engineered bacteria Escherichia.coli
Of BamHI.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs
GB/T 6682 analytical laboratory water specifications and experimental methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
BamH I
A restriction endonuclease that specifically recognizes the base sequence on the DNA strand G ↓ GATCC produces 5 'sticky ends derived from
Bacilus amyloliquefaciens H or Bacilus subtilis MT-2 (pBamHI RM22) or a recombinant E. coli strain containing
BamHI gene cloned from Bacilus amyloliquefaciens H strain.
3.2
BamHI activity unit BamH I activityunit
The amount of enzyme required to completely decompose 1 μg of λDNA was 1 viable unit (1 U) at 37 ° C for 1 h in a 50 μL reaction.
4 technical requirements
4.1 appearance
Clear and transparent liquid, no sediment.
4.2 enzyme activity
≥10000U/mL.
4.3 Impurities
It should not contain other endonucleases and exonucleases.
5 test methods
5.1 appearance
Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.
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