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GB/T 35540-2017: Restriction thrombin
Status: Valid
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| GB/T 35540-2017 | English | 199 |
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Restriction thrombin
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GB/T 35540-2017
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PDF similar to GB/T 35540-2017
Standard similar to GB/T 35540-2017 GB/T 41907 GB/T 35541 GB/T 35539 Basic data | Standard ID | GB/T 35540-2017 (GB/T35540-2017) | | Description (Translated English) | Restriction thrombin | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,190 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Regulation (derived from) | National Standards Bulletin 2017 No. 32 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35540-2017: Restriction thrombin---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Restriction thrombin
ICS 07.080
C27
National Standards of People's Republic of China
Restrictive thrombin
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Preface Ⅰ
Introduction Ⅱ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 technical requirements
5 test methods 1
6 packaging, transportation and storage 2
7 shelf life 2
Appendix A (Normative) Restrictive thrombin enzyme activity test 3
Appendix B (Normative) Restricted thrombin purity detection 5
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized.
This standard was drafted unit Fujian Huacan Pharmaceutical Co., Ltd., Huacan Nansheng (Xiamen) Biotechnology Co., Ltd., Fujian Nansheng Science and Technology Co., Ltd.
Company, Shandong University, Angel Yeast Co., Ltd., Sanming City Product Quality Inspection Institute, Fujian Agriculture and Forestry University, Fudan University, Suzhou University,
Shanghai 100 赛 Biological Technology Co., Beaver Nano Technology (Suzhou) Co., Ltd., Shanghai Boshi Biological Technology Co., Ltd.
The main drafters of this standard. Huangfa Can, Zheng Dengzhong, Zhan Xuexiong, Guo Xiaoxi, Chen Xiulan, Zhao Jing, Zhang Lili, Zhuang Liqiong, Huang Fa,
Lin Yue Chao, Chen Jinchun, Yao Juan, Liu Bin, Zhang Xiying, Zhong Jiang, Zhu Li, Ren Hui, Xing Zhigang.
Introduction
Restrictive thrombin is a highly specific serine protease that recognizes the amino acid sequence of LeuValProArg ↓ GlySer
Column, a genetically engineered recombinant fusion protein cutting tool. The development of national standards for restrictive thrombin to promote the type of enzyme tools industry tools
It is of great significance for the production and use of restricted thrombin.
Restrictive thrombin
1 Scope
This standard specifies the technical requirements of restrictive thrombin, test methods, packaging, transportation, storage and shelf life.
This standard applies to the restriction of blood from pig extract thrombin.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
A highly specific serine protease that recognizes the LeuValProArg ↓ GlySer amino acid sequence for digestion with
Recombinant fusion proteins of thrombin cleavage sites.
3.2
20 mmol/L Tris-HCl, 0.15 mol/L NaCl, 2.5 mmol/L NaCl
, The amount of limiting thrombin required to hydrolyze 1 μmol of fusion protein within 16 h is a Facanunit.
4 technical requirements
4.1 appearance
White or off-white lyophilized cake or powder.
4.2 enzyme activity
≧ 350Facanunit/mg.
4.3 Purity
20μg restriction thrombin by SDS-PAGE electrophoresis showed a single band.
5 test methods
5.1 appearance
Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.
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