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| GB/T 35029-2018 | English | 159 |
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Method based the microarray for mutation detection of hereditary hearing loss
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GB/T 35029-2018
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Basic data
| Standard ID | GB/T 35029-2018 (GB/T35029-2018) |
| Description (Translated English) | Method based the microarray for mutation detection of hereditary hearing loss |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | C30 |
| Classification of International Standard | 11.040.55 |
| Word Count Estimation | 8,878 |
| Date of Issue | 2018-05-14 |
| Date of Implementation | 2018-12-01 |
| Regulation (derived from) | National Standards Announcement No. 6 of 2018 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 35029-2018: Method based the microarray for mutation detection of hereditary hearing loss
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Genetic deafness gene detection method based on microarray chip)
ICS 11.040.55
C30
National Standards of People's Republic of China
Genetics based on microarray chips
Deafness gene detection method
Published on.2018-05-14
2018-12-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This standard is proposed and managed by the National Biochip Standardization Technical Committee (SAC/TC421).
This standard was drafted. Boao Biological Group Co., Ltd.
The main drafters of this standard. Jiang Di, Xiang Guangxin.
Genetics based on microarray chips
Deafness gene detection method
1 Scope
This standard specifies the method of detection of common hereditary deafness genes based on microarray chips.
This standard applies to clinically assisted diagnosis, neonatal screening, epidemiological investigation, healthy person screening and other fields of hereditary deafness
Detection of genetic loci.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 27990-2011 basic terminology of biochip
YY/T 1153 DNA microarray chip for in vitro diagnosis
YY/T 1154 laser confocal scanner
3 Terms and definitions
The terms and definitions defined in GB/T 27990-2011 apply to this document.
4 Abbreviations
The following abbreviations apply to this document.
DNA. deoxyribonucleic acid
dATP. deoxyadenosine triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate)
dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate)
dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)
GJB2. gap junction protein subunit 2 (gapjunctionbeta2)
GJB3. gap junction protein subunit 3 (gapjunctionbeta3)
PCR. polymerase chain reaction (polymerase chain reaction)
SDS. sodium dodecyl sulfate (sodiumdodecylsulfate)
SLC26A4. solute transporter 26 member 4 (solutecarrierfamily26, member4)
SSC. citrate buffer solution (citratebuffersolution)
5 Principle
According to the genetic locus gene mutation site information that has been found and confirmed based on the epidemiological investigation of rickets in China, multiple
Amplification and chip hybridization techniques detect genetic locus mutations commonly found in Chinese populations.
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