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GB/T 34796-2017 English PDF

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GB/T 34796-2017: Quantification and purity analysis of nucleic acid concentration in solution -- Ultraviolet spectrophotometry
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PDF similar to GB/T 34796-2017


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Basic data

Standard ID GB/T 34796-2017 (GB/T34796-2017)
Description (Translated English) Quantification and purity analysis of nucleic acid concentration in solution -- Ultraviolet spectrophotometry
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 9,966
Date of Issue 2017-11-01
Date of Implementation 2018-05-01
Regulation (derived from) National Standard Announcement 2017 No. 29
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34796-2017: Quantification and purity analysis of nucleic acid concentration in solution -- Ultraviolet spectrophotometry


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Quantification and purity analysis of nucleic acid concentration in solution-Ultraviolet spectrophotometry ICS 07.080 A40 National Standards of People's Republic of China Concentration and purity of nucleic acids in aqueous solution Detection of UV spectrophotometry Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard drafting unit. China Institute of Testing Technology Institute of Biology, Shanghai Institute of Measurement and Testing Technology, China Testing Technology Research Institute, Sichuan Zhongbian Biotechnology Co., Ltd. The main drafters of this standard. Tan peace, Zhou Lihua, Xu Li, Liu Gang, Ma Li Xia, Li Yan, Ye Deping, Liang Wen, Wang Zhi, Ding Min, Sun Dengfeng. Concentration and purity of nucleic acids in aqueous solution Detection of UV spectrophotometry

1 Scope

This standard specifies the principle of using UV spectrophotometry to detect the concentration and purity of nucleic acids in aqueous solution, sample preparation and testing methods. This standard applies to the concentration and purity of nucleic acids in aqueous solution testing, the concentration of 5ng/μL and above nucleic acid solution in aqueous solution Volume detection and purity analysis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods GB/T 11165 Laboratory pH meter GB/T 26813 dual-beam UV-visible spectrophotometer JJG646 pipette test procedures

3 Abbreviations

The following abbreviations apply to this document. DNA. Deoxyribonucleic acid RNA (ribonucleic acid)

4 principle

Nucleotides purine and pyrimidine base conjugated double bonds, with the nature of absorbing ultraviolet light, the maximum absorption at a wavelength of 250nm ~ Between 270nm. Base and pentose, phosphoric acid nucleotides form the maximum absorption peak will not change the maximum absorption wavelength of about 260nm, absorption Low peak at 230nm. At a wavelength of 260 nm, an optical density of OD value of 1 unit corresponds to a 50 μg/mL double-stranded DNA concentration, a single strand DNA and RNA is 40μg/mL, single-stranded oligonucleotide is 33μg/mL, the use of this amount and concentration relationship, can be used to calculate the nucleic acid sample concentration.

5 Reagents and materials

Unless otherwise specified, the reagents used in this method are of analytical grade, water is a water GB/T 6682 provisions. 5.1 DNA standard solution Calf thymus DNA standard solution. 5.2 Tris-buffer (1 mol/L Tris-HCl, pH 7.4) Weigh 121.1gTris placed in 1L beaker, add 800mL of deionized water, stir to dissolve, adjust the pH value with NaOH,

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