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GB/T 34738-2017: Method of the real-time PCR for the detection of honeybees sacbrood disease
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 34738-2017 | English | 239 |
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Method of the real-time PCR for the detection of honeybees sacbrood disease
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GB/T 34738-2017
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Basic data | Standard ID | GB/T 34738-2017 (GB/T34738-2017) | | Description (Translated English) | Method of the real-time PCR for the detection of honeybees sacbrood disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 12,145 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34738-2017: Method of the real-time PCR for the detection of honeybees sacbrood disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method of the real-time PCR for the detection of honey bees sacbrood disease
ICS 11.220
B41
National Standards of People's Republic of China
Fluorescent PCR Detection of Honeybee Cysticercosis
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted unit Jilin Entry-Exit Inspection and Quarantine of People's Republic of China, Jilin University, People's Republic of China Fujian Entry and Exit
Inspection and Quarantine Bureau.
The drafters of this standard. Song war Yun, Feng Xin, Meng Rising, Liu Jinhua, Wei Chunyan, Mu Jun, Wang Zhenguo, Zheng Teng, Meng Qingfeng, Cai Yang, Xiao Cheng Rui,
Wang Weili.
Fluorescent PCR Detection of Honeybee Cysticercosis
1 Scope
This standard specifies the bee larvae cytoplasmic fluorescence PCR detection method.
This standard applies to bees and larvae in cystic larval disease detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 18088 exit quarantine animal samples
3 Reagents and materials
Unless otherwise specified, only analytical reagents are used. All reagents were treated with RNase-free containers (treated with diethyl pyrocarbonate
After autoclave) dispensing.
3.1 water, GB/T 6682, three.
3.2 bee larvae virus.
3.3 Primers and Probes, Upstream Primer F1 (10 pmol/μL) .5'-AAGTTGGAGGCGCGYATTTG-3 ', Downstream Primer
R1 (10 pmol/μL) .5'-CAAATGTCTTCTTACDAGAAGYAAGGATTG-3 ', probe P1 (5 pmol/μL).
5'-FAM-CGGAGTGGAAAGAT-TAMRA-3 '.
3.4 phosphate buffered saline, the preparation method see A.1.
3.5 Total RNA extraction reagent.
3.6 chloroform.
3.7 isopropanol.
3.8 75% ethanol.
Note. Prepare with freshly opened absolute ethanol and diethyl pyrocarbonate.
3.9 Coke-diethyl carbonate treated water.
3.10 reverse transcriptase.
3.11 RNase inhibitor (40U/μL).
3.12 5 × reverse transcriptase buffer.
3.13 10 × PCR reaction buffer (containing magnesium ions).
3.14 DNA Polymerase (5U/μL).
3.15 Deoxyribonucleotide triphosphates (dNPTs 10 mmol/L each).
4 equipment
4.1 fluorescence quantitative PCR amplification instrument.
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