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GB/T 34736-2017: Detection of jaagsiekte sheep retrovirus with dot blotting hybridization
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Detection of jaagsiekte sheep retrovirus with dot blotting hybridization
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GB/T 34736-2017
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Basic data | Standard ID | GB/T 34736-2017 (GB/T34736-2017) | | Description (Translated English) | Detection of jaagsiekte sheep retrovirus with dot blotting hybridization | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 15,143 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34736-2017: Detection of jaagsiekte sheep retrovirus with dot blotting hybridization---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of jaagsiekte sheep retrovirus with dot blotting hybridization
ICS 11.220
B41
National Standards of People's Republic of China
Detection of Sheep Lung Adenovirus Virus by Dot Assay
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB T1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
The main drafting units of this standard. Inner Mongolia Agricultural University, People's Republic of China Exit Inspection and Quarantine, Beijing Agricultural Vocational
College.
The drafters of this standard. Liu Shuying, Qi Jingwei, Wang Haiyan, Wang Zhenling, Ma Xueen, Liang Huachun, Zhao Zeyun, Sun Xiaolin.
Introduction
Ovinepora pulmonary adenomatosis (OPA) is a disease caused by the sheep lung adeno-virus (Jaagsiektesheepret-
rovirus, JSRV) is a chronic, progressive and contagious lung neoplasm disease. The main feature is the sheep cough, breathing
Difficult, a large number of serous nasal fluid, weight loss, type Ⅱ alveolar epithelial cells and ciliated bronchial epithelial neoplasia [1]. Autopsy can be seen
Sheep lung consolidation, retraction poor, larger, larger than the normal lung 2 to 3 times. The surface of the lungs have a solid circular miliary size gray
Color nodules that adenoma, nodules occur fusion, the formation of irregular shape and large nodules. Airway filled with foamy liquid, lifted
Two hind legs can flow out of the nostril foamy liquid that is a card-based cart test [2].
Sheep lung adeno-virus (JSRV) is the causative agent of sheep pulmonary adenoma. Contained within all genomes of normal sheep that have been studied
There are about 20 copies of endogenous retroviruses (ERVs) that are closely related to JSRV. Because of their gene
The structure is very similar to that of sheep lung adenoma virus, also known as the endogenous sheep lung adenovirus (enJSRV), in order to distinguish between sheep lung adenoma
The virus is exJSRV [3]. Studies have shown that the gene structure of enJSRV and exJSRV are highly conserved among the gag gene, long terminal repeat (LTR)
env gene at a larger difference, especially env gene encoding transmembrane protein region (TM) and long terminal repeat U3 region is 2 high
Variable area. Therefore, in the detection of pathogen exJSRV, specific primers were designed at these two hypervariable regions and specific probes were prepared to exclude
enJSRV [4] [5].
So far, no single method of diagnosing the disease has been established in the world [1]. There are three reasons for this. One is the potential of the disease
Volt longer, longer duration, in the incubation period or obvious clinical symptoms of sheep is difficult to find. Second, ill sheep do not detect circulating anti-body
Body, it is difficult to use conventional immunological methods for diagnosis, and third is the pathogen caused by the disease of sheep lung adeno-virus (JSRV) has not yet established in vitro
Raising system, so conventional virus isolation and identification methods can not be used. The current diagnosis of the disease also depends mainly on clinical symptoms and pathology
Weave examination. Therefore, the establishment of specific diagnostic criteria for the disease in the country's entry and exit inspection and quarantine, domestic sheep safety assessment, sheep inspection
Epidemic mass and so has a very important significance.
Detection of Sheep Lung Adenovirus Virus by Dot Assay
1 Scope
This standard specifies the sheep lung adenomatous virus nucleic acid dot blot hybridization reagents, materials and equipment, sampling, methods of operation, the results of determination.
This standard applies to the detection of clinical suspected sick sheep and import and export of sheep lung tissue or peripheral blood samples of sheep lung adeno-associated virus nucleic acid, also
Suitable for rapid detection of sheep lung adenomatosis pathogens in sheep clinical samples.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Terms and Definitions, Abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Dot blotting dotblottinghybridization
The nucleic acid probe is hybridized to the DNA sample to be tested on a hybrid membrane using a nucleic acid probe with a suitable label for post-reaction detection
It binds to a specific target molecule to form a hybrid, which is then visualized by a color reaction. Dot blotching is to be tested directly sheep tissue or blood-like
DNA denaturation, spotted on the nitrocellulose membrane or nylon membrane, and then with the preparation of specific probes hybridization to detect the sample before
Molecular hybridization of viral DNA.
3.2 Abbreviations
The following abbreviations apply to this document.
AMV. Avian Myeloblastosis Virus
BCIP 5-Bromo-4-Chloro-3-Indolyl-Phosphate
cDNA. Complementary DNA
DNA. Deoxyribonucleic Acid
dNTPs.4 kinds of deoxyribonucleoside triphosphate mixture (Deoxyribonucleoside Triphosphates)
EDTA. Ethylenediamine tetraacetic acid (EthylenediaminetetraaceticAcid)
enJSRV. Endogenous JatrophaeStepRetrovirus
ERV. Endogenous Retrovirus
JSRV. Sheep lung adenoma virus (JaagsiekteSheepRetrovirus)
NBT. Nitro-Blue-Tetrazolium
OPA. Ovine Pulmonary Adenomatosis
PCR. Polymerase Chain Reaction (Polymerase Chain Reaction)
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