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GB/T 32947-2016: Determination of octacosanol and triacontanol contents in beeswax -- Gas chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 32947-2016 | English | 119 |
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Determination of octacosanol and triacontanol contents in beeswax -- Gas chromatography
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GB/T 32947-2016
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Basic data | Standard ID | GB/T 32947-2016 (GB/T32947-2016) | | Description (Translated English) | Determination of octacosanol and triacontanol contents in beeswax -- Gas chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B47 | | Classification of International Standard | 65.140 | | Word Count Estimation | 6,623 | | Date of Issue | 2016-08-29 | | Date of Implementation | 2017-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Announcement 2016 No.14 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the determination of octacosanol and triacontanol in beeswax. This standard applies to the determination of octacosanol and triacontanol in beeswax. | GB/T 32947-2016: Determination of octacosanol and triacontanol contents in beeswax -- Gas chromatography
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Determination of octacosanol and triacontanol contents in beeswax.Gas chromatography
ICS 65.140
B47
National Standards of People's Republic of China
Determination of Octacosanol and Triacontanol in Beeswax
Gas chromatography
2016-08-29 released
2017-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the All-China Federation of Supply and Marketing Cooperatives.
This standard is the national bee product standardization working group.
The drafting of this standard. Institute of Bee Research, Chinese Academy of Agricultural Sciences, Ministry of Agriculture Bee Product Quality Supervision, Inspection and Testing Center (Beijing), China
Beekeeping Society.
The main drafters of this standard. Li Yi, Xue Xiaofeng, Huang Jingping, Wu Jie, Wu Liming, Zhao Jing, Zhang Jinzhen, Zhou Jinhui, Chen Fang, Wang Peng, Chen Lanzhen.
Determination of Octacosanol and Triacontanol in Beeswax
Gas chromatography
1 Scope
This standard specifies the determination of octacosanol and triacontanol in beeswax.
This standard is applicable to the determination of octacosanol and triacontanol in beeswax.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 principle
After the sample was hydrolyzed by reflux, the octacosanol and triacontanol in the sample were extracted with chloroform and the extract was dried over anhydrous sodium sulfate
Chemical and gas chromatographic flame ionization detector detection, external standard method quantitative.
4 Reagents and Materials
4.1 Unless otherwise stated, analytical pure reagents are used and water is the primary water specified in GB/T 6682.
4.2 Potassium hydroxide.
4.3 anhydrous sodium sulfate. Incubate at 650 ° C for 4 h before use.
4.4 Trichloromethane.
4.5 95% ethanol.
4.6 0.5mol/L potassium hydroxide ethanol solution; weighed 2.8g potassium hydroxide, with 100mL 95% ethanol dissolved in plastic bottles, room temperature
Sealed to save.
4.7 octacosanol, triacontanol standards. purity ≥ 98%.
4.8 octacosanol, triacontol standard stock solution. were weighed 20mg octacosanol, triacontanol standard, accurate to 0.1mg,
Into the 100mL volumetric flask, add trichloromethane ultrasonic dissolved after volume. Sealed at room temperature.
4.9 octacosanol, triacontol standard working solution. accurately absorb the standard stock solution 0.50mL, 1.00mL, 2.50mL, 5.00mL,
10.00 mL in a 10 mL volumetric flask, formulated with trichloromethane at 10.0 mg/L, 20.0 mg/L, 50.0 mg/L, 100.0 mg/L,
200.0mg/L standard working solution. Sealed at room temperature.
5 equipment
5.1 Gas Chromatograph. equipped with a flame ionization detector (FID).
5.2 ultra-low temperature refrigerator.
5.3 high-speed grinding grinder.
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