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GB/T 32946-2016: Determination of proline in honey -- High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 32946-2016 | English | 119 |
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Determination of proline in honey -- High performance liquid chromatography
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GB/T 32946-2016
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Basic data | Standard ID | GB/T 32946-2016 (GB/T32946-2016) | | Description (Translated English) | Determination of proline in honey -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X31 | | Classification of International Standard | 67.180.10 | | Word Count Estimation | 6,695 | | Date of Issue | 2016-08-29 | | Date of Implementation | 2017-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Announcement 2016 No.14 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the content of proline in honey by high performance liquid chromatography. This standard applies to the determination of the amount of proline in honey. | GB/T 32946-2016: Determination of proline in honey -- High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of proline in honey.High performance liquid chromatography
ICS 67.180.10
X31
National Standards of People's Republic of China
Determination of Proline in Honey by High Performance Liquid Chromatography
2016-08-29 released
2017-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the All-China Federation of Supply and Marketing Cooperatives.
This standard is the national bee product standardization working group.
The drafting of this standard. Institute of Bee Research, Chinese Academy of Agricultural Sciences, Ministry of Agriculture Bee Product Quality Supervision, Inspection and Testing Center (Beijing), China
Beekeeping Society.
The main drafters of this standard. Li Yi, Zhang Jinzhen, Huang Jingping, Wu Jie, Wu Liming, Zhao Jing, Xue Xiaofeng, Zhou Jinhui, Chen Fang, Wang Peng, Chen Lanzhen.
Determination of Proline in Honey by High Performance Liquid Chromatography
1 Scope
This standard specifies the content of proline in honey by high performance liquid chromatography.
This standard applies to the determination of proline content in honey.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 method summary
The proline in the sample was extracted with boric acid buffer, derivatized by column, liquid chromatographic fluorescence detector, external standard method.
4 reagents and materials
4.1 Unless otherwise stated, analytical reagents are used and water is the primary water specified in GB/T 6682.
4.2 Methanol. Chromatographic purity.
4.3 acetonitrile. pure chromatography.
4.4 tetrahydrofuran. chromatographic purity.
4.5 Sodium acetate.
4.6 Acetic acid.
4.7 boric acid.
4.8 disodium ethylenediaminetetraacetate.
4.9 Sodium hydroxide.
4.10 phosphoric acid.
4.11 hydrochloric acid.
4.12 L-proline standard (CAS number. 147-85-3). purity ≥99%.
4.13 4-Fluoro-7-nitro-2,1,3-benzoxathiadiazole (NBD-F, CAS. 29270-56-2). purity ≥99%.
4.14 sodium hydroxide solution (0.1mol/L). Weigh 2.00g sodium hydroxide, dissolved in 500mL of water, mix, transfer to the polyethylene bottle,
Closed place.
4.15 Sodium acetate buffer (0.1 mol/L). Weigh 13.60 g of sodium acetate, dissolve in 1 L of water, adjust the pH to 7.2 with acetic acid,
Degassed.
4.16 boric acid buffer (0.1mol/L). Weigh ethylenediamine tetraacetic acid disodium 0.186g, boric acid 3.09g, dissolved in 500mL water, hydrogen
Sodium hydroxide solution adjusted to pH 8.0.
4.17 hydrochloric acid solution (0.1mol/L). take 8.33mL hydrochloric acid, constant volume to 1L.
4.18 NBD-F solution (100mmoL/L). Weigh NBD-F0.0183g in a brown liquid phase vial, add 1mL acetonitrile dissolved,
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