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GB/T 32945-2016: Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro
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| GB/T 32945-2016 | English | 439 |
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Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro
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GB/T 32945-2016
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Basic data | Standard ID | GB/T 32945-2016 (GB/T32945-2016) | | Description (Translated English) | Bovine tuberculosis diagnosis -- Assay on IFN-�� in vitro | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,182 | | Date of Issue | 2016-08-29 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | National Standard Announcement 2016 No.14 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 32945-2016: Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Bovine tuberculosis diagnosis - Assay on IFN-γ in vitro
ICS 11.220
B41
National Standards of People's Republic of China
In vitro detection of bovine tuberculosis by interferon - gamma method
2016-08-29 released
2017-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Directory
Preface I
Introduction II
1 Scope 1
2 Abbreviations 1
Principle 1
4 IFN-γ in vitro assay 1
4.1 Instruments and supplies 1
4.2 Reagent 2
4.3 Procedure 2
Appendix A (normative) Reagent preparation 4
Appendix B (informative) Sampling and sample handling precautions 6
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuer of this document does not assume responsibility for the identification of these patents.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China.
This standard is nationalized by the National Animal Health Standardization Technical Committee (SAC/TC181).
The drafting of this standard. Huazhong Agricultural University, Wuhan Branch before the animal biological products limited liability company, Beijing Entry-Exit Inspection and Quarantine Bureau, wide
Animal Disease Prevention and Control Center of West Zhuang Autonomous Region.
The drafters of this standard. Guo Aizhen, Chen Yingyu, Tu Lingling, Hu Changmin, Chao Jin, Ma Guiping, Hu Qiaoyun, Chen Huanchun.
Introduction
Bovine tuberculosis is mainly caused by Mycobacterium bovis (Mycobacterium bovis, M. bovis) caused by a zoonotic chronic infectious diseases,
Showing a worldwide distribution. The World Organization for Animal Health (OIE) lists it as a must-report and international trade must be classified as
Second-class animal disease, is the "national long-term animal disease prevention and control plan (2012 ~ 2020)" 16 kinds of priority control of the domestic major animal disease
one. Clinical tuberculosis is the most common type of tuberculosis, but breast tuberculosis and intestinal tuberculosis can also occur. Healthy cattle can be contaminated by
Air, feed and drinking water through the respiratory and gastrointestinal infections; In addition, cattle tuberculosis is one of the important sources of human tuberculosis. As the cattle branch
Bacteria infection spectrum widely, persistent infection, cattle slow course of tuberculosis, clinical symptoms such as atypical reasons, cattle tuberculosis purification is relatively difficult. along with
In recent years, China's modern dairy industry and the rapid development of beef cattle industry and domestic cattle trade increasingly frequent, China's cattle tuberculosis is on the rise.
Domestic and foreign generic bovine tuberculosis control strategy is "quarantine - culling", therefore, accurate detection is the premise of control and purification of bovine tuberculosis.
The current quarantine method for bovine tuberculin intradermal allergy, the method has the advantage of high sensitivity, the disadvantage is. the operation time-consuming cumbersome, knot
Fruit judgment is subjective and can be cross-reactive with environmental mycobacterial infections, immunosuppressive or tuberculosis reactivity to tuberculin
reduce. Bovine tuberculosis diagnosis in vitro detection of IFN-γ method is the use of bovine tuberculin and tuberculin to stimulate peripheral blood lymphocyte release
IFN-γ, a method for determining the infection status of bovine tuberculosis by detecting IFN-γ concentration is a method with high sensitivity and specificity,
And can achieve high-throughput detection. Developed countries have successfully used this method to assist in the diagnosis and monitoring of bovine tuberculosis. Establishment of China 's Bovine Tuberculosis Diagnosis
IFN-γ in vitro test national standard, for cattle tuberculosis early diagnosis, quarantine, monitoring and epidemiological survey to provide technical support, to promote
Into the control and purification of bovine tuberculosis in China, and ensure the healthy and sustainable development of cattle industry in China.
In vitro detection of bovine tuberculosis by interferon - gamma method
1 Scope
This standard specifies the abbreviations, principles, reagents, sampling and operating procedures for the detection of gamma tuberculosis in vitro by the detection of bovine tuberculosis.
This standard applies to the early diagnosis, quarantine, surveillance and epidemiological investigation of bovine tuberculosis.
2 abbreviations
The following abbreviations apply to this document.
A-PPD. avianpurifiedproteinderivative
B-PPD. bovinepurifiedproteinderivative
ELISA. enzymoinked immunosorbent assay (enzymelinkedimmunosorbentassay)
HRP. horseradishperoxidase
IFN-γ. interferonamma
IgG. immunoglobulin G
PBS. phosphate buffer (phosphatebuffersolution)
OD. optical density
3 principle
Mycobacterium tuberculosis positive tuberculosis, its peripheral blood lymphocytes have been Mycobacterium bovis sensitized and produce immune memory. When in vitro
In the event of bovine mycobacterial specific antigen (bovine tuberculin) stimulation, lymphocytes will be activated, the occurrence of re-cellular immune response,
Put IFN-γ. Mycobacterium bovis infection can be diagnosed by detecting the level of IFN-γ by sandwich ELISA. Mycobacterium tuberculosis is non-tuberculosis
Mycobacterium tuberculosis, with tuberculin to stimulate lymphocytes as a control, can effectively rule out the environment caused by mycobacterial infection nonspecific reaction.
4 IFN-γ in vitro assay
4.1 Instruments and supplies
4.1.1 Class II biological safety cabinet.
4.1.2 Carbon dioxide incubator.
4.1.3 Microplate reader.
4.1.4 Mixer.
4.1.5 refrigerator.
4.1.6 balance.
4.1.7 high pressure disinfection pot.
4.1.8 trace adjustable pipette and tip.
4.1.9 Disposable sterile centrifuge tube (2 mL).
4.1.10 disposable heparin sodium anticoagulant blood collection tube.
4.1.11 Disposable syringes (5 mL).
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