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GB/T 32268-2015: Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column
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| GB/T 32268-2015 | English | 399 |
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Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column
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GB/T 32268-2015
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Basic data | Standard ID | GB/T 32268-2015 (GB/T32268-2015) | | Description (Translated English) | Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | N04 | | Classification of International Standard | 71.040 | | Word Count Estimation | 19,164 | | Date of Issue | 2015-12-10 | | Date of Implementation | 2016-07-01 | | Quoted Standard | GB/T 6682; GB/T 9008; GB/T 32267; JJG 705 | | Regulation (derived from) | National Standard Announcement 2015 No.38 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the performance of octadecyl bonded phase high performance liquid chromatography ("C18 HPLC column"). This standard applies to the determination of the performance of C18 HPLC columns. | GB/T 32268-2015: Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column
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Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column
ICS 71.040
N04
National Standards of People's Republic of China
Octadecyl bonded phase (C18) HPLC column
Performance measurement method
Methodofperformancetestingforoctadecylbonded (C18) highperformance
liquidchromatography (HPLC) column
Issued on. 2015-12-10
2016-07-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard was proposed by the Ministry of Science and Technology of the People's Republic of China.
This standard by National Instrument Analysis and Testing Standardization Technical Committee (SAC/TC481) centralized.
This standard is drafted by. Tianjin Science and Technology Co., Ltd. 博纳艾杰尔.
Participated in the drafting of this Standard. China Association for Instrumental Analysis, Peking University, Institute of Chemistry, Chinese Quantitative Research
Institute, Chinese Academy of Food and Drug test, Beijing Nonferrous Metal Research Institute, Waters Technology (Shanghai) Co., Ltd., Dalian Elite Analyzer
Co., Ltd, Beijing Jin Ouya Technology Development Co., Ltd.
The main drafters of this standard. Wang Wan, Wang Qun-Jie, Zhao Yu, Zhang Xueyun, together Zhang, Liu Quan Liu Huwei, Hu Changqin, Wang Zhengfan, Caikuai Xia,
Wang Ruomei.
Octadecyl bonded phase (C18) HPLC column
Performance measurement method
1 Scope
This standard specifies the octadecyl bonded phase HPLC column (referred to as the "C18HPLC pillar") determination of properties.
This standard applies to the determination of C18HPLC column performance.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 9008 term liquid chromatography column chromatography and planar chromatography method
GB/T 32267 Determination term performance of the instruments
JJG705 liquid chromatography test procedures
3 Terms and Definitions
GB/T 9008 and GB/T 32267 and defined by the following terms and definitions apply to this document.
3.1
Octadecyl bonded phase (C18) HPLC column octadecylbondedhighperformanceliquidchromatography
column
Equipped with octadecyl bonded stationary phase HPLC column.
3.2
Column diameter columninternaldiameter
Column column pipe inner diameter.
3.3
Hydrophobic retention factor retentionfactorofhydrophobicity
αn
Characterization C18 stationary phase relative to the number of parameters C18 alkyl chains, and the specific surface area and the surface carbon coverage of filler particles is concerned.
3.4
Hydrophobic selectivity factor selectivityfactorofhydrophobicity
αhp
Characterization of C18 stationary phase was separated parameter substance molecule hydrophobic group selective recognition of, and filler particles surface carbon coverage
related.
3.5
Stereoselectivity factor stericselectivityfactor
αss
Parameters characterizing the ability to identify substances to be separated C18 stationary phase molecular shape of the surface of the filler particles with a functional group key degree
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