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GB/T 31802-2015: Detection and identification of Tomato spotted wilt virus
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| GB/T 31802-2015 | English | 319 |
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Detection and identification of Tomato spotted wilt virus
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GB/T 31802-2015
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Basic data | Standard ID | GB/T 31802-2015 (GB/T31802-2015) | | Description (Translated English) | Detection and identification of Tomato spotted wilt virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 15,140 | | Date of Issue | 2015-07-03 | | Date of Implementation | 2015-11-20 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | National Standard Announcement 2015 No.22 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the method for identification of quarantine phytosanitary Tomato spotted wilt virus. This Standard applies to quarantine and identification of plant seeds, seedlings and other propagation material of tomato spotted wilt virus. | GB/T 31802-2015: Detection and identification of Tomato spotted wilt virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Tomato spotted wilt virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Tomato spotted wilt virus quarantine and identification methods
Issued on. 2015-07-03
2015-11-20 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. MATURAL, Lu Jie, Ma Jie, hole Jun, Wang Xiufen, Du Zhixin, Zhang Yongjiang, Weimei Sheng.
Tomato spotted wilt virus quarantine and identification methods
1 Scope
This standard specifies the method for identification of quarantine phytosanitary Tomato spotted wilt virus.
This standard applies to quarantine and identification of plant seeds, seedlings and other propagation material of tomato spotted wilt virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 equipment and reagents
3.1 Equipment
Microplate reader, the balance (a sense of volume 1/10000g), pH meter, trace juicer, PCR, real-time PCR instrument, electrophoresis, electrophoresis tank,
Gel imaging system, isolate the greenhouse, water bath, cold refrigerator.
Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), ELISA plate, mortar tubes, flower pots, sterilization
Soil.
3.2 Reagents
Enzyme-linked immunosorbent assay reagent see Appendix B, RT-PCR detection reagent in Appendix C, real-time fluorescent RT-PCR detection reagent see
Appendix D, biological assay reagent see Appendix E.
4 Sampling and Sample Preparation
4.1 Sampling
Sampling conducted in accordance with the provisions of SN/T 2122's.
4.2 Preparation of seed samples
Seeds (emphasis picked malformation, immature seed) sown in sterilized soil, grown and symptoms observed at 25 ℃. Grow to be 3 ~
4 leaves, the plants will show symptoms of number of plants did not show symptoms of the packet (10 as a group) and number. Leaves collected for enzyme-linked immunosorbent
Determination, by RT-PCR, real-time fluorescence RT-PCR, and biological assays.
4.3 Preparation of the sample seedlings
The seedlings were grown in a greenhouse in isolation, growth and symptoms observed at 25 ℃. Symptomatic seedlings individually tested. No symptoms of sub
Group testing, grouping method and detection methods with 4.2.
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