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GB/T 31792-2015: Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic Mihaljcevic et Petrov
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| GB/T 31792-2015 | English | 319 |
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Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic Mihaljcevic et Petrov
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GB/T 31792-2015
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Basic data | Standard ID | GB/T 31792-2015 (GB/T31792-2015) | | Description (Translated English) | Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic Mihaljcevic et Petrov | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 15,185 | | Date of Issue | 2015-07-03 | | Date of Implementation | 2015-11-27 | | Regulation (derived from) | National Standard Announcement 2015 No.22 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 31792-2015: Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic Mihaljcevic et Petrov
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Detection and identification of Diaporthe helianthi Muntanola-Cvetkovic Mihaljcevic et Petrov
ICS 65.020.01
B16
National Standards of People's Republic of China
Sunflower stem canker quarantine and identification methods
Issued on. 2015-07-03
2015-11-27 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Chinese Academy of Inspection and Quarantine, China National Accreditation Center, the Xinjiang People's Republic of China
Entry Inspection and Quarantine Bureau.
Drafters of this standard. Wu Shan goods, Du Hongzhong, Cailou Min, Zhang Xianglin, Yan Jin.
Sunflower stem canker quarantine and identification methods
1 Scope
This standard specifies the sunflower stem canker quarantine authentication methods.
This standard applies to plant sunflower seeds and sunflower stem canker quarantine and identification of bacteria.
2 apparatus and appliances
2.1 Instrument
Stereo microscope, biological microscope, clean benches, light incubator, electronic scales (1/1000g), autoclaves, refrigerators, PCR
Amplification, fluorescence PCR amplification device, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath.
2.2 Appliances
Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle
Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank, sample screen (10 mesh, φ1.7mm).
3 Reagents and culture medium
3.1 Reagents
Glucose, streptomycin, lactic acid, agar, 1.0% sodium hypochlorite (NaClO4), liquid nitrogen, Tris-HCl, MgCl2, HCl, EDTA, NaOH,
NaOAc, KCl, Tris, CTAB, TAE, ethanol (Ethanol), chloroform (Chloroform), isopropyl alcohol (Isopropanol), iso
Amyl alcohol (Isoamylalcohol), SYBRGreenⅠ nucleic acid dyes, proteinase K, TaqDNA polymerase, dNTP, universal primers ITS5
And ITS4, real-time PCR primers DH-F, DH-R probe and DH-Pr.
3.2 Medium
Potato dextrose agar (PDA), streptomycin potato dextrose agar medium (SPDA). See attached specific formula
Record A.
4 identification of quarantine
4.1 Symptom Check
Field collection stems suspicious symptoms, diseased leaves or sunflower plate, clip the junction of healthy tissue disease backup material. Typical symptoms of the disease stems
Ulcers and petiole light brown spots, see Appendix A description of the symptoms of disease.
Selected in the seed shriveled, discolored, wrinkled, torn or moldy seed, while the selection of sunflower entrained debris, such as petioles, stems
Rod, disk debris.
4.2 Isolation
You would have suspected symptoms of leaves, petioles, stems, and other debris disk sample clip 5mm ~ 10mm pieces at the junction of disease and health, with
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