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GB/T 31793-2015 English PDF

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GB/T 31793-2015: Detection and identification of Leptosphaeria maculans (Fuckel) Ces.et De Not.
Status: Valid
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GB/T 31793-2015English319 Add to Cart 3 days [Need to translate] Detection and identification of Leptosphaeria maculans (Fuckel) Ces.et De Not. Valid GB/T 31793-2015

PDF similar to GB/T 31793-2015


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Basic data

Standard ID GB/T 31793-2015 (GB/T31793-2015)
Description (Translated English) Detection and identification of Leptosphaeria maculans (Fuckel) Ces.et De Not.
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.010
Word Count Estimation 15,110
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31793-2015: Detection and identification of Leptosphaeria maculans (Fuckel) Ces.et De Not.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Leptosphaeria maculans (Fuckel) Ces.et De Not. ICS 65.020.010 B16 National Standards of People's Republic of China Rapeseed stem canker quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Shanghai Exit Inspection and Quarantine. Drafters of this standard. Wu Shan goods, Yi Jianping, Zhou Guoliang, Du Hongzhong. Rapeseed stem canker quarantine and identification methods

1 Scope

This standard specifies the rapeseed stem canker quarantine authentication methods. This standard applies to canola and rapeseed stem canker and other cruciferous host plants rapeseed seed stem canker quarantine And identification.

2 apparatus and appliances

2.1 Instrument Electronic balance (inductance 0.01g), biological microscopes, stereo microscope, high-speed refrigerated centrifuge, water meter, clean bench, autoclave Pot, ice maker, PCR, gel imaging system, electrophoresis, real-time PCR instrument, incubator, refrigerator. 2.2 Appliances Beakers, flasks, graduated cylinder, dish, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, mortar, pipettes, shift Dispenser tips, centrifuge tubes, liquid nitrogen tank, aperture 2.5mm and 1.5mm mesh screen.

3 Reagents and culture medium

3.1 Reagents 1.0% sodium hypochlorite (NaClO4), agar, potato, glucose, lactate, agarose, sterile double distilled water, liquid nitrogen, peptone, dicalcium phosphate Potassium, magnesium sulfate, streptomycin, neomycin, PCNB, chlortetracycline, rifampicin, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAc, KCl, Tris, CTAB, TAE, absolute ethanol (ethanol), phenol (phenol), ethidium bromide (EB), chloroform (chloro- form), isopropyl alcohol (isopropanol), iso-amyl alcohol (isoamylalcohol), proteinase K, TaqDNA polymerase, dNTP, DNA relative Molecular weight standards, PCR primers and real-time PCR primers and probes. 3.2 Medium Potato dextrose agar (PDA), potato dextrose agar medium acidic (APDA). See Appendix A. specific formula

4 identification of quarantine

4.1 seed testing Seed with seed not, dissecting microscope picked wrinkled, shriveled, discolored, deformed, incomplete or moldy seed 1g ~ 2g; with seed Seed coating agents, first washed with tap water and soak seed coating, or wrapped with gauze soaked seed scrub, rinse water to the seed coating to be dried after Picked abnormal seed, so isolated and cultured. Commercial canola samples taken 1kg, with a pore size of 2.5mm and 1.5mm mesh sieve, the pore size of 2.5mm sieve and was mostly pods Stems and other plant debris, 1.5mm aperture sieve material and debris, mostly small rapeseed. The sieve material and sieve material mix, take 1g ~ 2g system Thus, PCR was carried out the first test, the first test if positive, then picked abnormal isolated and cultured seed do.

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