|
US$319.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 31801-2015: Detection and identification of Phytophthora syringae Kleb. Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 31801-2015 | English | 319 |
Add to Cart
|
3 days [Need to translate]
|
Detection and identification of Phytophthora syringae Kleb.
| Valid |
GB/T 31801-2015
|
PDF similar to GB/T 31801-2015
Basic data | Standard ID | GB/T 31801-2015 (GB/T31801-2015) | | Description (Translated English) | Detection and identification of Phytophthora syringae Kleb. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 15,190 | | Date of Issue | 2015-07-03 | | Date of Implementation | 2015-11-27 | | Regulation (derived from) | National Standard Announcement 2015 No.22 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
GB/T 31801-2015: Detection and identification of Phytophthora syringae Kleb.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora syringae Kleb.
ICS 65.020.01
B16
National Standards of People's Republic of China
Quarantine and identification of Phytophthora syringae
Issued on. 2015-07-03
2015-11-27 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Tianjin, People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Du Hongzhong, Roca Feng, Wu Shan goods, Yan Jin.
Quarantine and identification of Phytophthora syringae
1 Scope
This standard specifies the method for inspection and identification of Phytophthora syringae.
This standard applies to the host for Phytophthora syringae seedlings, branches, leaves, fruits and entrained soil and medium cloves Phytophthora
Identification.
2 apparatus and appliances
2.1 Instrument
Biological microscope, clean benches, light incubator, electronic scales (1/1000g), autoclaves, refrigerators, PCR amplification, fluorescence
PCR amplification, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath.
2.2 Appliances
Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle
Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank.
3 reagents, materials and media
3.1 Reagents
β- sitosterol, pimaricin (Pimaricin), ampicillin sodium salt (Ampicilin), sodium salt rifampicin (rifampicin,), five clonazepam
Benzene (pentachloronitrobenzene), hymexazol (hymexazol), CaCO3, agarose, Tris-HCl, MgCl2, HCl, edetate
Acid tetrasodium salt (EDTA), NaOH, NaOAc, KCl, Tris, cetyl trimethyl ammonium bromide (CTAB), TAE, ethanol (etha-
nol), chloroform (chloroform), isopropyl alcohol (isopropanol), iso-amyl alcohol (isoamylalcohol), SYBRGreenI nucleic acid dyes,
Proteinase K, TaqDNA polymerase, dNTP, universal primers ITS5 and ITS4, real-time PCR primer PS-F and PS-R and Taq-
Man-MGB probe PS-Pr.
3.2 Material
Agar, V8 juice, summer oak blades or apple or pear, orange skin or in leaves.
3.3 Medium
PDA medium, V8 medium, V8 medium orange leather, V8 leaves of medium, selective medium PARPH-V8. Equipment
See Appendix A. Matrix Formulation
4 identification of quarantine
4.1 Symptom Check
Check the host plant roots, stems, leaves, fruits and other parts, to have withered, fruit rot, root rot, crown rot symptoms and flow of plastic plants, as well as around the root
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 31801-2015_English be delivered?Answer: Upon your order, we will start to translate GB/T 31801-2015_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 31801-2015_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 31801-2015_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|