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GB/T 31791-2015: Detection and identification of Cotton leaf curl virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 31791-2015 | English | 259 |
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Detection and identification of Cotton leaf curl virus
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GB/T 31791-2015
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Basic data | Standard ID | GB/T 31791-2015 (GB/T31791-2015) | | Description (Translated English) | Detection and identification of Cotton leaf curl virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 12,190 | | Date of Issue | 2015-07-03 | | Date of Implementation | 2015-11-27 | | Regulation (derived from) | National Standard Announcement 2015 No.22 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the immunology and molecular biology methods such as quarantine and identification of cotton leaf curl virus. This Standard applies to quarantine and identification may be carrying cotton leaf curl virus in plant tissue. | GB/T 31791-2015: Detection and identification of Cotton leaf curl virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cotton leaf curl virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Cotton leaf curl virus quarantine and identification methods
Issued on. 2015-07-03
2015-11-27 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Chinese Academy of Inspection and Quarantine, Guangdong, People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
State and Yunnan Entry-Exit Inspection and Quarantine Xinjiang People's Republic of China, Zhejiang University, Shenzhen People's Republic of China
Entry-Exit Inspection and Quarantine Maoming People's Republic of China.
The main drafters. Zhang, Zhang Yongjiang, Feng Lixia, Min Li, Zhang Xianglin, Zhou Xueping, Zheng Yun, Ma Jie, Li Hailin, Xin Yan Yan,
Water Zhu Fang, Li Mingfu.
Cotton leaf curl virus quarantine and identification methods
1 Scope
This standard specifies the immunology and molecular biology methods such as quarantine and identification of cotton leaf curl virus.
This standard applies to quarantine and identification may be carrying cotton leaf curl virus in plant tissue.
2 equipment, appliances and reagents
2.1 Equipment
Electronic analytical balance (0.001g), a small centrifuge, refrigerated centrifuge desktop, heated water bath, the Reader, general PCR, quantitative fluorescence
Light PCR, electrophoresis system, pH meter, gel imaging system, 4 ℃ refrigerator, clean benches, -80 ℃ ultra-low temperature refrigerators, Autoclave,
Ice maker, a vortex, and microwave ovens.
2.2 Appliances
Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL), pipette tips, centrifuge tubes, Eppendorf tubes (0.2mL,
0.5mL, 1.5mL) and mortar and the like.
2.3 Reagents
Unless otherwise stated, all test reagents were analytical grade or biochemical reagents.
DAS-ELISA, conventional PCR and real-time PCR detection reagent, respectively, see Appendix B, Appendix C and Appendix D.
Quarantine and identification of 3
3.1 DAS-ELISA detection
DAS-ELISA detected in Appendix B.
3.2 conventional PCR detection
Conventional PCR detection Appendix C.
3.3 real-time PCR detection
Real-time PCR assay in Appendix D.
Analyzing the results of 4
Results 3.1, 3.2 and 3.3 in two different principles of detection methods were positive, the sample can be determined as CLCuV positive. Generally
DAS-ELISA test is positive, the conventional PCR or real-time PCR assay results can be judged positive samples CLCuV
Positive.
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