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GB/T 31730-2015: Detection of genetically modified components in rice -- Membrane-based array method
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| GB/T 31730-2015 | English | 299 |
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Detection of genetically modified components in rice -- Membrane-based array method
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GB/T 31730-2015
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Basic data | Standard ID | GB/T 31730-2015 (GB/T31730-2015) | | Description (Translated English) | Detection of genetically modified components in rice -- Membrane-based array method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 14,170 | | Date of Issue | 2015-06-02 | | Date of Implementation | 2015-09-01 | | Quoted Standard | GB/T 6682; GB/T 19495.1; GB/T 19495.2; GB/T 19495.3; GB/T 19495.6; GB/T 19495.7 | | Regulation (derived from) | National Standard Announcement 2015 No.19 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the method for the detection of rice and processed products in GMO rice. This Standard applies to BAR - transgenic herbicide-tolerant and Bt transgenic rice (CrylAb/CrylAc) rapid qualitative detection of genetically modified insect-resistant rice, and also applies to the rapid qualitative detection of processed products in more than rice ingredients. This Standard specifies the minimum detection limit of GMO is 1 g/kg. | GB/T 31730-2015: Detection of genetically modified components in rice -- Membrane-based array method
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Detection of genetically modified components in rice. Membrane-based array method
ICS 67.050
X04
National Standards of People's Republic of China
Determination of genetically modified rice film chip method
Issued on. 2015-06-02
2015-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard by the China National Institute of Standardization.
This standard was drafted. Sichuan Hua Han three biotechnology Ltd., China National Institute of Standardization, Heilongjiang Provincial Cereals, Oils health inspection supervisor
ST.
The main drafters of this standard. Huang Guangping, cloud Zhenyu, Yin Zhijian, Hu Taigui, SUN Zhao, Zhang Yao, Yongqiang, Dong Jiu red, Songting Rui, Zhang Jinlong,
Liu Zhen.
Determination of genetically modified rice film chip method
1 Scope
This standard specifies the method for the detection of rice and rice genetically modified ingredients in processed products.
This standard applies to BAR - transgenic herbicide-tolerant rice and transgenic Bt (Cry1Ab/Cry1Ac) insect-resistant genetically modified rice
Rapid qualitative detection, but also for the rapid qualitative detection of processed rice products above ingredients.
This standard specifies the minimum detection limit of GMO is 1g/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 19495.1 GMO testing - General requirements and definitions
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.3 GMO detection of nucleic acid extraction and purification method
GB/T 19495.6 GMO detection microarray detection method
GB/T 19495.7 GMO detection methods of sampling and sample preparation
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
GB/T 19495.1, terms and definitions GB/T 19495.6 defined apply to this document.
3.2 Acronyms
The following abbreviations apply to this document.
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
dATP. triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate)
dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate)
dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)
dUTP. deoxyuridine triphosphate (deoxyuridinetriphosphate)
bp. base pairs (basepair)
BAR. glufosinate acetyltransferase gene (phosphinothricinacetyltransferasegene)
Cry1Ab. Bacillus thuringiensis insecticidal toxin protein cry1A (b) gene [Bacillusthuringiensisinsecticidaltoxin
cry1A (b) gene]
Cry1Ac. Bacillus thuringiensis insecticidal toxin protein cry1A (c) gene [Bacillusthuringiensisinsecticidaltoxin
cry1A (c) gene]
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