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GB/T 45556-2025: Determination of pentosan content in plant-derived products - Gas chromatography/mass spectrometry method
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Determination of pentosan content in plant-derived products - Gas chromatography/mass spectrometry method
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GB/T 45556-2025
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Basic data | Standard ID | GB/T 45556-2025 (GB/T45556-2025) | | Description (Translated English) | Determination of pentosan content in plant-derived products - Gas chromatography/mass spectrometry method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 14,134 | | Date of Issue | 2025-04-25 | | Date of Implementation | 2025-11-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 45556-2025: Determination of pentosan content in plant-derived products - Gas chromatography/mass spectrometry method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.050
CCSX04
National Standard of the People's Republic of China
Determination of Pentosan Content in Products of Plant Origin
GC-MS
Released on 2025-04-25
2025-11-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the China National Institute of Standardization.
This document was drafted by. Tianjin Renewable Resources Research Institute of China National Supply and Marketing Cooperatives, Tianjin Ecological Environment Monitoring Center, Shenzhen
Brand Building Promotion Center, China National Institute of Standardization, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Tianjin University of Technology, China
Jiliang University, Yunnan Yunke Specialty Plant Extraction Laboratory Co., Ltd., Institute of Process Engineering, Chinese Academy of Sciences, Rizhao Quality Inspection and Testing
Research Institute, Shanxi Agricultural University, Lujian Testing Technology Co., Ltd., and Nanjing Zhongke Pharmaceutical Co., Ltd.
The main drafters of this document are. Li Man, Wang Yanli, Zhao Bin, Gui Jianye, Fei Ting, Zhang Li, Zhao Yunlong, Xi Xingjun, Cui Zhaojie, Wu Yufeng, Ge Yinling,
Liu Fude, Zi Chenrui, Lan Tao, Song Li, Ma Zheng, Zhang Jing, Gao Youkun, Li Jiangyan, Chen Chen, Gao Kai, Du Tao, Liu Lina, Chen Yisheng, Kong Yingjun, Zhao Yuhai,
Liu Dianjia, Ma Xiao, He Yan, Jiao Nianqiang, Liu He, Feng Peng, Zhou Yajie.
Determination of Pentosan Content in Products of Plant Origin
GC-MS
Warning---The derivatization reagents used in the experiment contain toxic substances. The sample derivatization process should be carried out in a fume hood.
Please wear protective equipment and avoid contact with skin and clothing.
1 Scope
This document describes a method for the determination of pentosan content in products of plant origin by gas chromatography-mass spectrometry.
This document applies to pentosans in wheat and bran, sorghum, millet, rice, soybeans, tea, sugar cane, pineapple peel, coconut milk, reed root, and edible fungi.
Determination of content. Other plant-derived products shall be implemented in accordance with the same regulations.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Pentosan
A condensation product of pentose (five-carbon sugar).
Note. The pentosan content in this document is calculated as the sum of arabinose and xylose.
4 Principle
Plant-derived products use water and saturated barium hydroxide solution as extractants to extract water-soluble pentosans or water-insoluble pentosans, respectively.
The product was hydrolyzed by xylanase to obtain arabinose and xylose as the main hydrolysis products. Hydroxylamine hydrochloride, pyridine and acetic anhydride were used as derivatization reagents.
The arabinose and xylose derivatives were obtained by solid phase extraction column purification and quantitative determination by gas chromatography-mass spectrometry.
The sum of the arabinose and xylose contents generated by the hydrolysis of sugars and water-insoluble pentosans was converted into the pentosan content in the plant-based product.
5 Reagents and Materials
Unless otherwise stated, all reagents used in this method were of analytical grade.
5.1 Reagents
5.1.1 Barium hydroxide [Ba(OH)2, CAS No.. 17194-00-2].
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