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GB/T 28873-2012 English PDF

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GB/T 28873-2012: General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles
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Basic data

Standard ID GB/T 28873-2012 (GB/T28873-2012)
Description (Translated English) General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A60
Classification of International Standard 17.180
Word Count Estimation 9,982
Quoted Standard GB/T 19619-2004; GB/T 21636-2008; GB/T 23414-2009
Regulation (derived from) National Standards Bulletin 2012 No. 28
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the environmental scanning electron microscopy nanoparticle interactions with biological samples and technical specifications. This standard applies to the application of low vacuum scanning electron microscopy and environmental va

GB/T 28873-2012: General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles ICS 17.180 A60 National Standards of People's Republic of China Morphology of the biological effects of nano particles Environmental scanning electron microscope detection method General Issued on. 2012-11-05 2013-02-15 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard was proposed by the Chinese Academy of Sciences. This standard by the National Standardization Technical Committee of nano technology (SAC/TC279) centralized. This standard is drafted by. National Center for Nanoscience. It participated in the drafting of this standard. Institute of Electrical Engineering, Second Military Medical University. The main drafters of this standard. Han Dong, Han, Chen Peipei, Xie instrument, Yong Ji, Yin Bohua, Sunquan Mei, Chu Weiguo, the early Ming Zhang, Jin Lin, Li Jing. Morphology of the biological effects of nano particles Environmental scanning electron microscope detection method General Warning. This standard does not point out all possible security issues. Before applying this standard, the user's responsibility to take appropriate safety and Health practices and to ensure compliance with the conditions relevant national regulations.

1 Scope

This standard specifies the technical specifications and environmental scanning electron microscopy of nanoparticles with a biological sample interactions. This standard applies to low vacuum mode of application and environmental vacuum scanning electron microscope to study the biological effects of nanoparticles in biological Analysis of the morphology of the sample (range be extended to sub-micron particles and micron).

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 19619-2004 nanomaterials term GB/T 21636-2008 Micro cytometry analysis electron probe microanalysis (EPMA) term GB/T 23414-2009 micro cytometry analysis Scanning Electron Microscopy term

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Environmental Scanning Electron Microscope environmentalscanningelectronmicroscope; ESEM In its work, the pressure in the sample chamber between 1Pa ~ 5000Pa change. Therefore, it can not directly detect the secondary electron emission Son, the image is enlarged by detecting electron gas, environmental scanning electron detector to detect ions, the photoelectric effect or other detection signal (such as Backscattered electrons or sink current) is obtained. 3.2 Biological effects of topography biologicaleffectontopography After the nanoparticles, biological sample morphology changes. 3.3 Low vacuum mode lowvacuummode In the high pressure vacuum tube, and the sample chamber at 10Pa ~ 130Pa, the surface of the sample for testing. 3.4 Environmental vacuum mode environmentalvacuummode In the high pressure vacuum tube, and the sample chamber is in the range 10Pa ~ 4000Pa, the detection surface of the sample. 3.5 Aqueous biological sample hydrousbiologicalsample Surface without the presence of excess water, internal water, easily deformed fresh samples.

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