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GB/T 27640-2011: Diagnostic techniques for horsepox disease
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27640-2011 | English | 259 |
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Diagnostic techniques for horsepox disease
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GB/T 27640-2011
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Basic data | Standard ID | GB/T 27640-2011 (GB/T27640-2011) | | Description (Translated English) | Diagnostic techniques for horsepox disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,158 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-04-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 22 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the horse pox clinical diagnosis, virus isolation and identification requirements and other diagnostic techniques. This standard applies to horse pox diagnosis. | GB/T 27640-2011: Diagnostic techniques for horsepox disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for horsepox disease
ICS 11.220
B41
National Standards of People's Republic of China
Horse pox Diagnosis
Issued on. 2011-12-30
2012-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. People's Republic of China Shandong Exit Inspection and Quarantine, China Animal Health and Epidemiology Center.
The main drafters of this standard. Liang beads, China Zhu, Xiao Xi Zhi, Zheng Xiaolong, Deng Mingjun, Yue Zhiqin, Bidao Rong, Liang Jun Ni, Xu Biao.
Horse pox Diagnosis
1 Scope
This standard specifies the horse pox and other clinical diagnosis, virus isolation and identification of diagnostic technical requirements.
This standard applies to horse pox diagnosis.
2 Clinical examination
2.1 Clinical features
Pimples skin disease in horses and Departments at the ball joint, and then become blisters, pustules and finally, and dry and crusted. Due to local pain
Pain, can cause lameness, the horse disease is generally not significant systemic symptoms; smallpox occurred some sick horses on the inside of the lips, gums, tongue, tongue tie, buccal mucosa, etc.,
Also starting papules, then vesicles and pustules, pustules rupture forming shallow ulcers; females genitalia edema, and the formation of water on the skin and mucous membranes
Blisters, pustules and ulcers, lesions showed sire penis, prepuce and pox-like lesions on the glans penis.
2.2 preliminary determination
2.2.1 preliminary determination sick horses horse pox infection may be made according to the clinical characteristics.
2.2.2 The final decision should be carried out virus isolation and identification.
3 virus isolation
3.1 Sample collection and delivery requirements
Take the lesion exudate, blister fluid and tissue ulcer after collection as an object, as far as possible collected aseptically collected cold air experiment
Room for virus isolation.
3.2 virus isolation test
3.2.1 the required instruments and equipment
3.2.1.1 CO2 incubator.
3.2.1.2 Ⅱ level biological safety cabinet.
3.2.1.3 inverted microscope.
3.2.1.4 centrifuge with refrigeration capabilities.
3.2.2 Material preparation
3.2.2.1 cell culture medium DMEM.
3.2.2.2 Hela cells.
3.2.2.3 Hanks solution (Hanks solution, see A.1; containing antibiotics, see A.4).
3.2.2.4 calf serum.
3.2.2.5 horse tissue to be tested. Take lesion exudates, liquid or pimples blisters and ulcers tissue as a specimen, formulated as a liquid with Hanks
1.10 suspension to 1500r/min centrifugal 10min, supernatant spare.
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