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GB/T 27637-2011 English PDF

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GB/T 27637-2011: Real-time PCR method for the detection of Mycobacterium avium subsp. paratuberculosis
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GB/T 27637-2011English259 Add to Cart 3 days [Need to translate] Real-time PCR method for the detection of Mycobacterium avium subsp. paratuberculosis Valid GB/T 27637-2011

PDF similar to GB/T 27637-2011


Standard similar to GB/T 27637-2011

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Basic data

Standard ID GB/T 27637-2011 (GB/T27637-2011)
Description (Translated English) Real-time PCR method for the detection of Mycobacterium avium subsp. paratuberculosis
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 11,138
Date of Issue 2011-12-30
Date of Implementation 2012-04-01
Quoted Standard GB/T 6682-2008; GB 19489-2008
Regulation (derived from) Announcement of Newly Approved National Standards No. 22 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the Mycobacterium paratuberculosis (Mycobacterium avium subsp. paratuberculosis) real-time PCR detection method of the technical requirements and operational specifications. This standard applies to quick detection of culture, blood and milk samples, faeces and tissue samples and other clinical Mycobacterium paratuberculosis.

GB/T 27637-2011: Real-time PCR method for the detection of Mycobacterium avium subsp. paratuberculosis


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for the detection of Mycobacterium avium subsp.paratuberculosis ICS 11.220 B41 National Standards of People's Republic of China Mycobacterium paratuberculosis real-time PCR detection method Issued on. 2011-12-30 2012-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, Jilin Agricultural University, Beijing Science and Technology Development has surplus Jiusi Limited. The main drafters of this standard. Liuzhong Yong, Chen Ru, Yang Guohai, Gao Yun Hang, Bi Zeng Jian, Zhu Road, Wu Xiaowei, Gao Bo. Mycobacterium paratuberculosis real-time PCR detection method

1 Scope

This standard specifies the Mycobacterium paratuberculosis (Mycobacteriumaviumsubsp.paratuberculosis) real-time PCR detection Technical requirements and test methods practices. This standard applies to quick detection of clinical cultures, blood, milk samples, faeces and tissue samples such as Mycobacterium paratuberculosis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682-2008 Analysis GB 19489-2008 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. Fluorescent signal cycle number reaches a set threshold when experienced. Ct value. dNTP. deoxyribonucleosidetriphosphate, deoxynucleoside triphosphates. DMSO. dimethylsulfoxide, dimethyl sulfoxide. EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid. PBS. phosphatebuffersolution, phosphate buffer. PCR. polymerasechainreaction, polymerase chain reaction. Taq enzyme. TaqDNA polymerase. UDG enzyme. uracilDNAglycosylase, uracil DNA glycosylase. Principle 4 This method uses a standard probe TaqMan real-time PCR technology principle. The reaction system includes one pair for the amplification of DNA Primer, and a PCR product hybridized specifically with the fluorescently labeled probe. Probe 5 'end labeled with a fluorescent reporter group is called Su, 3 'end labeled quencher. When performing PCR extension reaction, Taq polymerase 5 'exonuclease activity of the 5' fluorophore from the probe Cutting down to the quencher group separate, so the instrument can detect the fluorescence signal 5 'fluorophore issued, part of PCR amplification Generating part of the fluorescent signal generated increase was accompanied. With the cumulative increase in the number of amplification cycles, the instrument detects the fluorescent signal The reaction changes the amount of amplification product. F57 gene sequence of the standard ways to Mycobacterium paratuberculosis-specific template, design-specific amplification Primers and probes, fluorescent in PCR reaction system.

5 Materials and Reagents

5.1 Instruments and Supplies 5.1.1 inoculation stick.

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