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GB/T 27634-2011: Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27634-2011 | English | 299 |
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Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
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GB/T 27634-2011
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Basic data | Standard ID | GB/T 27634-2011 (GB/T27634-2011) | | Description (Translated English) | Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 13,172 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-04-01 | | Quoted Standard | GB/T 6682; GB/T 18088; GB 19489 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 22 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the infectious bursal disease virus by RT-PCR method and the real-time fluorescence RT-PCR detection method of the technical requirements and operational specifications. This standard applies to infectious bursal disease virus infection in rapid diagnosis. | GB/T 27634-2011: Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
ICS 11.220
B41
National Standards of People's Republic of China
Infectious bursal disease virus nucleic acid detection methods
(Gumborodisease) virus
Issued on. 2011-12-30
2012-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, South China Agricultural
University, People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, Beijing Science and Technology Co., Ltd. surplus Jiusi.
The main drafters of this standard. Qinzhi Feng, Sun Jie, Lu Kang body, flower Qun Yi, Chen Shukun, Lv Jianqiang, Yang Su, Fang Xingwang, have less spirit, Zhan Aijun,
Bi Ying Zuo, Bing, Ruanzhou Xi, Zhu Yulan.
Infectious bursal disease virus nucleic acid detection methods
1 Scope
This standard specifies the technical requirements for infectious bursal disease virus by RT-PCR method and real-time RT-PCR detection methods and operations
For specification.
This standard applies to rapid diagnosis of infectious bursal disease virus infection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
GB 19489 General requirements for laboratory biosafety
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent signal cycle number reaches a set threshold when experienced. Ct value.
IBD. infectiousbursaldisease, infectious bursal disease.
IBDV. infectiousbursaldiseasevirus, infectious bursal disease virus.
SPF. specificpathogenfree, specific pathogen.
4 Materials and Reagents
4.1 Instruments and Supplies
4.1.1 DNA electrophoresis instrument.
4.1.2 gel imager.
4.1.3 refrigerated centrifuge (centrifugal force of 15000g up above).
4.1.4 Mixer.
4.1.5 refrigerator (2 ℃ ~ 8 ℃, -20 ℃, -80 ℃).
4.1.6 Micro adjustable pipette (10μL, 100μL, 1000μL).
4.1.7 Trace tape cartridge nozzle (10μL, 100μL, 1000μL).
4.1.8 sterilized mortar.
4.1.9 centrifuge tube.
Optical 4.1.10 PCR reaction tube.
4.1.11 PCR instrument.
4.1.12 real-time PCR instrument.
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