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GB/T 27531-2011: Protocol of reverse transcription polymerase chain reaction (RT-PCR) for the pathogen of viral encephalopathy and retinopathy
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27531-2011 | English | 259 |
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Protocol of reverse transcription polymerase chain reaction (RT-PCR) for the pathogen of viral encephalopathy and retinopathy
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GB/T 27531-2011
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Basic data | Standard ID | GB/T 27531-2011 (GB/T27531-2011) | | Description (Translated English) | Protocol of reverse transcription polymerase chain reaction (RT-PCR) for the pathogen of viral encephalopathy and retinopathy | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,160 | | Date of Issue | 2011-11-21 | | Date of Implementation | 2012-03-01 | | Quoted Standard | GB/T 6682; GB/T 18088 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 18 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the viral encephalopathy and retinopathy (viral encephalopathy and retinopathy, VER) pathogen isolation and reverse transcription polymerase chain reaction (RT-PCR) detection methods. This standard applies to VER pathogen epidemiology, diagnosis, quarantine and monitoring. | GB/T 27531-2011: Protocol of reverse transcription polymerase chain reaction (RT-PCR) for the pathogen of viral encephalopathy and retinopathy
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Protocol of reverse transcription polymerase chain reaction (RT-PCR) for the pathogen of viral encephalopathy and retinopathy
ICS 11.220
B41
National Standards of People's Republic of China
Viral encephalopathy and retinopathy pathogen RT - polymerization
Chain reaction (RT-PCR) detection method
Protocolofreversetranscriptionpolymerasechainreaction (RT-PCR)
Issued on. 2011-11-21
2012-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. People's Republic of China, Shenzhen CIQ.
The main drafters of this standard. Orientin Liu, Lu Kang body, He Junqiang, Shixiu Jie, Zhengxiao Cong, Jia Peng, Ye Yi gifted, Yang Jinshun.
Viral encephalopathy and retinopathy pathogen RT - polymerization
Chain reaction (RT-PCR) detection method
1 Scope
This standard specifies the viral encephalopathy and retinopathy (viralencephalopathyandretinopathy, VER) pathogen isolation and reverse
Transcription polymerase chain reaction (RT-PCR) detection method.
This standard applies to VER pathogens epidemiology, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Abbreviations
The following abbreviations apply to this document.
CPE. cytopathic (cytopathiceffect)
CTAB. cetyl trimethyl ammonium bromide (hexadecyltrimethyammoniumbromide)
DEPC. Coke ethylene carbonate (diethypyrocarbonate)
EB. ethidium bromide (ethidiumbromide)
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid)
HEPES. hydroxyethyl piperazine acetic sulfuric acid [4- (2-hydroxyerhyl) piperazine-1-erhanesulfonicacid]
RNA. ribonucleic acid (ribonucleicacid)
RNasin. Ribonuclease Inhibitor (ribonucleaseinhibitor)
VER. viral encephalopathy and retinopathy (viralencephalopathyandretinopathy)
4 Reagents and materials
4.1 Water. in line with GB/T 6682 in a water specifications, DEPC-treated to remove RNA enzyme.
4.2 VERV virus reference strains.
4.3 -EDTA trypsin digestion mix (see Appendix A, A.1).
4.4 L-15 medium (see Appendix A A.2).
4.5 SSN-1 cell line. with L-15 medium at 25 ℃.
4.6 E-11 cell line. with L-15 medium at 25 ℃.
4.7 AMV reverse transcriptase. 20U/μL, -20 ℃ to save, not to repeated freezing and thawing or extreme temperature changes.
4.8 RNasin. 20U/μL, -20 ℃ to save, not to repeated freezing and thawing or extreme temperature changes.
4.9 trypsin.
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