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GB/T 26517-2011: Determination of 24 preservative in cosmetics -- High performance liquid chromatography method
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| GB/T 26517-2011 | English | 209 |
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Determination of 24 preservative in cosmetics -- High performance liquid chromatography method
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GB/T 26517-2011
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Basic data | Standard ID | GB/T 26517-2011 (GB/T26517-2011) | | Description (Translated English) | Determination of 24 preservative in cosmetics -- High performance liquid chromatography method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 9,928 | | Date of Issue | 2011-05-12 | | Date of Implementation | 2011-10-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 6 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the twenty-four kinds of preservatives in cosmetics by HPLC side given method. This standard applies to creams, lotions, lotions and other skin care cosmetics category twenty-four kinds of preservatives determination. | GB/T 26517-2011: Determination of 24 preservative in cosmetics -- High performance liquid chromatography method
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Determination of 24 preservative in cosmetics. High performance liquid chromatography method
ICS 71.100.70
Y42
National Standards of People's Republic of China
Twenty-four kinds of preservatives in cosmetic determination
High Performance Liquid Chromatography
Issued on. 2011-05-12
2011-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard proposed by China National Light Industry Council.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard was drafted. Chinese Academy of Inspection and Quarantine, the Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of Spices, Shanghai
Quality Inspection and Technical Research Institute.
The main drafters of this standard. Wu Ting, Wang Chao, Ma Qiang, Xiao Haiqing, Zhang, Shen Min, Kang Wei.
Twenty-four kinds of preservatives in cosmetic determination
High Performance Liquid Chromatography
1 Scope
This standard specifies the cosmetics HPLC method twenty-four kinds of preservatives.
This standard applies to creams, lotions, water, and other skin care cosmetics category twenty-four kinds of preservatives in cosmetic determination.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Methanol as solvent, ultrasonic extraction, centrifugation, 0.45μm organic membrane filter, the solution was injected with a diode array detector
(DAD) liquid chromatography detection, external standard.
4 Reagents and materials
Unless otherwise specified, reagents were of analytical grade. Water as a water GB/T 6682 regulations.
4.1 Methanol. chromatographically pure.
4.2 salicylic acid, purity of not less than 99.0%; ethyl 4-hydroxybenzoate, purity of not less than 99.0%; propyl 4-hydroxybenzoate, the purity is not
Less than 99.0%; 4-hydroxybenzoic acid ester, a purity of not less than 99.0%; methyl 4-hydroxybenzoate, purity of not less than 99.0%; methyl isobutyl chloro
Thiazolidinone, purity of not less than 99.0%; methylisothiazolinone, purity of not less than 99.0%; benzyl alcohol, purity of not less than 99.0%; phenoxy
Ethanol, purity of not less than 99.0%; p- chloro -m- cresol, purity of not less than 98.0%; triclosan, purity of not less than 97.0%; triclocarban,
A purity of not less than 99.0%; benzoate, a purity of not less than 99.0%; ethyl benzoate, purity of not less than 99.0%; phenyl benzoate, pure
Not less than 99.0%; 2-bromo-2-nitropropane-1,3-diol, a purity of not less than 98.0%; 2,4-dichloro-3,5-dimethylphenol, purity of not less than
99.0%; 4-hydroxy benzoate, purity of not less than 99.0%; o- phenylphenol, purity of not less than 98.0%; chlorine xylenol, purity is not
Less than 98.0%; 4-hydroxy benzoate, isobutyl purity of not less than 99.0%; benzyl chlorophenol, purity of not less than 99.0%; p purity not less
To 99.0%; sorbic acid, purity of not less than 99.0%.
Note. 2,4-dichloro-3,5-di-p-cresol are not included in the supervision Wei Fa [2007] No. 1 "Hygienic Standard for Cosmetics" Table 4 "cosmetic components restricted preservative" in.
4.3 preservative Standard stock solution. Accurately weigh each preservative (4.2) 0.5g, accurate to 0.0001g, were placed in 50mL beaker, add
An appropriate amount of methanol dissolved and transferred to 100mL volumetric flask, diluted with methanol to the mark, mix, that was 5000mg/L of each standard stock preservative
Preparation of liquid.
4.4 preservative mixed standard stock solution. Pipette are standard stock solution of 2mL (4.3) to 100mL volumetric flask with methanol to
Scale, that was each preservative concentration was 100mg/L mixed standard stock solution. Preserve refrigerator can be used for three months.
4.5 Preservatives standard solution. methanol mixed standard stock solution (4.4) respectively dubbed a series of concentration of 0.5μg/mL,
1μg/mL, 5μg/mL, 10μg/mL, the standard working solution 20μg/mL, and stored in the refrigerator, may be used one week.
4.6 0.025mol/L sodium dihydrogen phosphate solution (pH3.80). Weigh sodium dihydrogen phosphate (NaH2PO4 · 2H2O) 3.9g, accurate to
0.001g, in a 50 mL beaker, dissolved in water and transferred to 1000 mL volumetric flask with water to volume, mix, that was
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