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GB/T 26425-2010: Method for determination of Clostridium perfringens in feeds
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26425-2010 | English | 349 |
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Method for determination of Clostridium perfringens in feeds
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GB/T 26425-2010
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Basic data | Standard ID | GB/T 26425-2010 (GB/T26425-2010) | | Description (Translated English) | Method for determination of Clostridium perfringens in feeds | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 15,140 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-07-01 | | Quoted Standard | GB/T 8170; GB/T 14699.1; GB/T 20195; SN/T 1538.1-2005; SN/T 1538.2-2007 | | Adopted Standard | ISO 7937-2004, MOD | | Regulation (derived from) | National Standard Approval Announcement 2011 No.2 (Total No.167) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the feed Clostridium perfringens testing methods. This standard applies to detect the feed of Clostridium perfringens. | GB/T 26425-2010: Method for determination of Clostridium perfringens in feeds---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for determination of Clostridium perfringens in feeds
ICS 65.120
B46
National Standards of People's Republic of China
Clostridium perfringens feed detection
(ISO 7937.2004, Microbiologyoffoodandanimalfeedingstuffs-
Colony-counttechnique, MOD)
Issued on. 2011-01-14
2011-07-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard uses redrafted law revision using ISO 7937.2004 "food and animal feed Microbiology Clostridium perfringens count
Horizontal method for counting the number of colonies of Technology "(in English).
This standard and ISO 7937.2004, compared with more adjustment in the structure, listed in Appendix B of this standard and ISO 7937.2004's
Reg number control list.
Technical differences exist compared to 2004, the terms of these differences has been involved through its outer margin bit. This standard and ISO 7937
Opposing vertical single line (︱) were identified in Appendix C gives the list of the corresponding technical differences and their causes.
This standard also made the following editorial changes.
--- Remove the ISO 7937.2004 The directory;
--- Remove the ISO 7937.2004 Introduction;
--- Remove the ISO 7937.2004 reference.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized.
This standard was drafted. Guangdong microbiological analysis of the test center.
The main drafters of this standard. Zhu Honghui, Sunxiao Tang, Song Yang Zhen, Chenyuan Liang, Zhang Jiao fresh.
Clostridium perfringens feed detection
1 Scope
This standard specifies the feed Clostridium perfringens test methods.
This standard applies to detect feed Clostridium perfringens.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 8170 repair value expressed about the rules and limit values \u200b\u200band judgment
GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT)
GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT)
SN/T 1538.1-2005 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
(SN/T 1538.1-2005, ISO /T S11133-1.2000, MOD)
SN/T 1538.2-2007 media preparation Guide Part 2. A Practical Guide to medium performance test (SN/T 1538.2-
2007, ISO /T S11133-2.2003, MOD)
Principle 3
3.1 plate inoculation specific amount of test fluid sample, or a specific amount of other types of test samples initial suspension. Under the same conditions to be vaccinated
Test sample or initial suspension tenfold dilutions thereof. Pouring a layer of selective medium, solidified and then pouring a layer of this medium coverage.
3.2 tablet anaerobic 20h ± 2h at 37 ℃.
3.3 typical colony counting.
3.4 Number of typical colonies with confirmatory tests confirmed that further calculations per milliliter or in the number of C. perfringens per gram sample.
4 dilution, media and reagents
Unless otherwise indicated in the analysis using only recognized as analytical reagents, laboratory water using distilled or deionized water, or equivalent
Purity water.
4.1 peptone saline diluent. A A.1 in the Appendix.
4.2 sulfite - cycloserine agar (SC). See Appendix A of A.2.
Note. The original medium called no yolk tryptone - sulfite - cycloserine agar (TSC).
4.3 fluid thioglycollate medium. A A.3 in the Appendix.
4.4 lactose sulfite (LS) medium (optional). A A.4 in the appendix.
4.5 Power nitrate media (optional). A A.5 in the Appendix.
4.6 nitrate reduction agent (optional). Refer to Appendix A in A.6.
4.7 zinc powder (optional). Optional.
4.8 Lactose - gelatin medium (optional). Refer to Appendix A A.7.
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