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GB/T 26351-2010: Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26351-2010 | English | 169 |
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Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica
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GB/T 26351-2010
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Basic data | Standard ID | GB/T 26351-2010 (GB/T26351-2010) | | Description (Translated English) | Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 7,754 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2011 No.129 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the German cockroach (Blattella germanica) in vivo insensitive acetylcholinesterase (AChE) detection method. This standard applies to German cockroach to organophosphate, biochemical detection of carbamate insecticides resistance. | GB/T 26351-2010: Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica
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Test methods of cockroach resistance to insecticides.Test methods of insensitive acetylcholinesterase for Blattella germanica
ICS 11.020
C05
National Standards of People's Republic of China
Detection method of cockroach resistance
German cockroach - insensitive acetylcholinesterase method
2011-01-14 release
2011-05-01 implementation
Ministry of Health of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and centralized by the Ministry of Health of the People's Republic of China.
The drafting of this standard. Beijing Municipal Center for Disease Control and Prevention, China Agricultural University, Chinese Academy of Military Medical Sciences
Institute of Epidemiology.
The main drafters of this standard. Zengxiao Peng, Gao Xiwu, Ma Yan, Qian Kun, Tong Ying, Liang Pei, Zhao Tongyan.
Detection method of cockroach resistance
German cockroach - insensitive acetylcholinesterase method
1 Scope
This standard specifies the method for the detection of insensitive acetylcholinesterase (AChE) in Blattellagermanica.
This standard is applicable to the biochemical test of the resistance of Blattella germanica to organophosphorus and carbamate.
2 terms and definitions
The following terms and definitions apply to this document.
2.1
Differentiation of acetylcholinesterase
Can inhibit the susceptibility of the German cockroach acetylcholinesterase activity 90% of the concentration of pesticides in mol/L or
mmol/L.
2.2
F1 generation F1generation
From the scene to capture the German cockroaches, after breeding the first generation.
3 test insects
Field Blattella germanica, indoor breeding, F1 generation after emergence 7d ~ 15d male German cockroaches. Ice bath under the conditions of storage does not exceed
1d, -20 ℃ under the conditions of storage is not more than 1 week or -80 ℃ under the conditions of storage within 6 months.
4 reagent
4.1 disodium hydrogen phosphate (Na2HPO4), chemically pure.
4.2 potassium dihydrogen phosphate (KH2PO4), chemically pure.
4.3 Triton X-100 (TritonX-100).
4.4 Acetylchiocholine (ATCh), analytical grade.
4.5 5,5'-dithiobisobenzoic acid (DTNB), analytical grade.
4.6 Phosphoric acid buffer (0.1 mol/L, pH 7.5 PBS). Weigh 30.18 g of Na2HPO4 · 12H2O and 2.16 g of KH2PO4,
Water volume to 1000mL.
4.7 DTNB-ethanol reagent. Weigh 12.4mgDTNB, dissolved in 125mL 95% ethanol, add 50mL phosphate buffer, the most
After the volume of distilled water to 250mL, 4 ℃ dark preservation.
4.8 ATCh solution. Weigh 28.9mgATCh dissolved in 10mL distilled water, -20 ℃ dark preservation.
4.9 ATCh-DTNB mixed solution. Weigh 75mgATCh and 13mgDTNB, dissolved in 100mL phosphate buffer.
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