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GB/T 26350-2010: Test methods of fly resistance to insecticides -- The bioassay methods for musca domestica
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26350-2010 | English | 169 |
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Test methods of fly resistance to insecticides -- The bioassay methods for musca domestica
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GB/T 26350-2010
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Basic data | Standard ID | GB/T 26350-2010 (GB/T26350-2010) | | Description (Translated English) | Test methods of fly resistance to insecticides -- The bioassay methods for musca domestica | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 7,736 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2011 No.2 (Total No.167) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the housefly (Musca domestica) organic phosphorus, carbamate, biological detection methods pyrethroid insecticide resistance. This standard applies to laboratory bioassays housefly resistance. | GB/T 26350-2010: Test methods of fly resistance to insecticides -- The bioassay methods for musca domestica
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test methods of fly resistance to insecticides. Bioassay methods for musca domestica
ICS 11.020
C05
National Standards of People's Republic of China
Flies resistance test method
Biomass assay of housefly
2011-01-14 release
2011-05-01 implementation
Ministry of Health of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and centralized by the Ministry of Health of the People's Republic of China.
The drafting of this standard. China Agricultural University, Beijing Center for Disease Control and Prevention, Chinese Academy of Military Medical Sciences Microbiology
Institute of Epidemiology.
The main drafters of this standard. Gao Xiwu, Zengxiao Peng, Ma Yan, Liu Ting, Liang Pei, Qian Kun, Zhao Tongyan.
Flies resistance test method
Biomass assay of housefly
1 Scope
This standard specifies the resistance of Muscadomestica to insecticidal agents such as organophosphorus, carbamate and pyrethroids.
Method of detection.
This standard applies to laboratory biology testing of housefly resistance.
2 terms and definitions
The following terms and definitions apply to this document.
2.1
Diagnostic dose
The amount of the pesticide dose calculated from the sensitive baseline of the susceptible strain corresponding to the mortality rate of 99% or 99.9%
99% or 99.9% of the susceptible strains were killed, and the dose was used to distinguish between resistant and sensitive individuals by biometric techniques.
2.2
F1 generation F1generation
From the scene to capture the housefly, after breeding the first generation.
2.3
Method topicalapplicationmethod
A certain amount of liquid directly drops in the specific part of the body wall of the insect to determine the pharmacy poison against the insects.
2.4
Membrane method medicalmembranemethod
Applying a quantity of the chemical solution to the surface of the carrier and forming a uniform film, and then placing the test substance on the carrier, the medicine passing through the insect
Foot, body wall and other insects into the body to play a toxic effect, after a certain time after the statistical mortality rate. Common carrier with filter paper, glass surface and so on.
3 test insects
Field breeding housefly, indoor breeding, feathering after 3d ~ 5d F1 female housefly.
4 reagent
Acetone or other organic solvents, CO2 or ether, the original drug.
5 instruments
Incubation boxes or rooms with controlled light and temperature, Tortoise cage, 100 mL serum vials, pipettes, micropumps (micro sampler).
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