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GB/T 23502-2009 English PDF

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GB/T 23502-2009: Determination of ochratoxin A in food -- High performance liquid chromatographic method with immunoaffinity column clean-up
Status: Obsolete
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GB/T 23502-2009English239 Add to Cart 3 days [Need to translate] Determination of ochratoxin A in food -- High performance liquid chromatographic method with immunoaffinity column clean-up Obsolete GB/T 23502-2009

PDF similar to GB/T 23502-2009


Standard similar to GB/T 23502-2009

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Basic data

Standard ID GB/T 23502-2009 (GB/T23502-2009)
Description (Translated English) Determination of ochratoxin A in food -- High performance liquid chromatographic method with immunoaffinity column clean-up
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 6,616
Date of Issue 2009-04-08
Date of Implementation 2009-05-01
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the ochratoxin A in food content immunoaffinity chromatography purification by high performance liquid chromatographic method. This standard applies to food and food products, butter and butter products-Determination of ochratoxin A content, sauce, , vinegar liquor. This standard method detection limit: Food and food products, the detection limit was 1. 0��g/kg, alcohol detection limit of 0. 1��g/kg, soy sauce, vinegar, butter and butter products detection limit of 0. 5��g/kg.

GB/T 23502-2009: Determination of ochratoxin A in food -- High performance liquid chromatographic method with immunoaffinity column clean-up


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Determination of ochratoxin A in food. High performance liquid chromatographic method with immunoaffinity column clean-up ICS 67.050 X04 National Standards of People's Republic of China Foods Determination of ochratoxin A in Immunoaffinity chromatography and HPLC purification Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Food Industry Standardization Technical Committee (SAC/TC64) proposed. This standard by the National Standardization Technical Committee on Food Universal Detection Technology Food Industry Technical Committee (SAC/TC64/SC8) Centralized. This standard was drafted. Qingdao City Product Quality Supervision and Inspection, People's Republic of China Shandong Exit Inspection and Quarantine, the inspection Wellcome Technology Co., Ltd., Tsingtao Brewery. The main drafters of this standard. Wu Rujun, Bao Lei, Zhang Huizhen, Li Huiying, Zhang Peng, Hung Yu Jun, Wang Xiong. Foods Determination of ochratoxin A in Immunoaffinity chromatography and HPLC purification

1 Scope

This standard specifies the food content of ochratoxin A immunoaffinity chromatography and HPLC purification method. This standard applies to the determination of food products, wine, soy sauce, vinegar, soy sauce and butter products of ochratoxin A in food and agriculture. The detection limit of this standard. food and food products, the detection limit was 1.0μg/kg, alcohol detection limit of 0.1μg/kg, soy sauce, Vinegar, soy sauce and sauce products, detection limit is 0.5μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

Extract sample was extracted with ochratoxin A, after immunoaffinity column purified by high performance liquid chromatography with fluorescence detector, external Standard method.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water line with GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. 4.3 glacial acetic acid. Chromatographic pure. 4.4 1 extract. methanol + water (80 + 20). 4.5 extract 2. Weigh 150g of sodium chloride, 20g of sodium bicarbonate dissolved in about 950mL of water, add water to 1L. 4.6 Rinse solution. Weigh 25g sodium chloride, 5g of sodium bicarbonate dissolved in about 950mL of water, add water to 1L. 4.7 Mycotoxins washing buffer. Weigh 25.0g NaCl, 5.0g of sodium bicarbonate dissolved in water was added 0.1mL Tween-20, washed with water Diluted to 1L. 4.8 ochratoxin A standard. Purity ≥98%. 4.9 Ochratoxin A stock standard solution. Weigh accurately a certain amount of ochratoxin A standard, dissolved in methanol and acetonitrile + (1 + 1), Dubbed 0.1mg/mL standard stock solution stored at -20 ℃, can be used for 3 months. 4.10 ochratoxin A standard working solution. Depending on needs, Imbibe ochratoxin A certain amount of stock solution, dilute with mobile phase Release, dubbed respectively equivalent to 1ng/mL, 5ng/mL, 10ng/mL, 20ng/mL, working standard solution 50ng/mL of, 4 ℃ preservation, can Use 7d. 4.11 ochratoxin A immunoaffinity column. 4.12 glass fiber filter. diameter 11cm, pore size 1.5μm, no fluorescent properties. 5. Apparatus 5.1 Balance. a sense of the amount of 0.001g.

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