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US$209.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 23196-2008: Determination of ferulic acid content in propolis -- HPLC-UV method Status: Valid
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Determination of ferulic acid content in propolis -- HPLC-UV method
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GB/T 23196-2008
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Basic data | Standard ID | GB/T 23196-2008 (GB/T23196-2008) | | Description (Translated English) | Determination of ferulic acid content in propolis -- HPLC-UV method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B47 | | Classification of International Standard | 65.140 | | Word Count Estimation | 9,999 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-06-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the propolis liquid chromatographic method ferulic acid. This standard applies to the determination of ferulic acid in propolis. The detection limit of this standard: 4 ��g/kg. |
GB/T 23196-2008: Determination of ferulic acid content in propolis -- HPLC-UV method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of ferulic acid content in propolis. HPLC-UV method
ICS 65.140
B47
National Standards of People's Republic of China
Determination of ferulic acid in propolis
LC - UV detection method
Posted 2008-12-31
2009-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard Annex A, Annex B and Annex C is informative appendix.
This standard is proposed and managed by China Federation of Supply and Marketing Cooperatives.
This standard was drafted. Australian Medicare Ling Pharmaceutical Co., Ltd. in Hangzhou, China Federation of Supply and Marketing Cooperatives bee products Standardization Technical Committee
Council, Chinese Academy of Agricultural Sciences Institute of bees, CIQ Jiangsu, Nanjing University, Jiangsu Laoshan biotechnology company.
The main drafters of this standard. Yuying Min, Hu Xiaolan, Zhu Jinliang, Xu Chengzhi, Zhou Jinhui, Li Gonghai, Chen Kun, Hong vibration training.
Determination of ferulic acid in propolis
LC - UV detection method
1 Scope
This standard specifies the liquid chromatographic method propolis ferulic acid.
This standard applies to the determination of ferulic acid in propolis.
The detection limit of this standard. 4μg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Propolis ferulic acid with methanol ultrasonic extraction, precipitation, centrifugation, and then the supernatant was 0.45μm membrane filtration by reverse phase column
After separation by liquid chromatography - ultraviolet detector measurement, external standard, based on the retention times and peak areas for qualitative and quantitative.
4 Reagents and materials
Unless otherwise specified, the reagents used were of analytical grade; water as a water GB/T 6682 provisions (distilled water or equivalent purity water); not
When specified with a solvent which formulated refers to an aqueous solution.
4.1 Methanol. chromatographically pure.
4.2 0.085% phosphoric acid solution (volume fraction). amount of 0.85mL phosphoric acid, water volume to 1000mL, through 0.45μm membrane filter.
4.3 acetonitrile. chromatography.
4.4 70% methanol (by volume). Measure 70mL of analytical grade methanol was added 30mL of water were mixed uniformly.
4.5 ferulic acid standard substance. purity ≥99%.
4.6 ferulic acid standard stock solution (200.0μg/mL). Weigh accurately 10.0mg (accurate to 0.1mg) ferulic acid standard substance (4.5)
In 50mL brown volumetric flask, add 70% methanol (4.4) to dissolve and dilute to volume, and mix, this solution can be at a temperature lower than 4 ℃ ice
Box refrigerated for two months.
4.7 ferulic acid standard solution. Pipette appropriate amount of ferulic acid standard stock solution (4.6) 1mL, 2.5mL, 5.0mL,
10.0mL, 20.0mL, 40.0mL, 80.0mL to 100mL volumetric flask, with 70% methanol (4.4) to the mark, dubbed
2.0μg/mL, 5.0μg/mL, 10.0μg/mL, 20.0μg/mL, 40.0μg/mL, 80.0μg/mL, 160.0μg/mL working standard
Solution, now with the current.
5 instruments and equipment
5.1 liquid chromatograph. equipped with a UV detector.
5.2 Analytical balance. accurate to 0.1mg.
5.3 membrane filter (polyvinylidene fluoride microporous membrane type F, 25mm). 0.45μm.
5.4 ultrasound.
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