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GB/T 23195-2008: Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 23195-2008 | English | 149 |
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Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry
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GB/T 23195-2008
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Basic data | Standard ID | GB/T 23195-2008 (GB/T23195-2008) | | Description (Translated English) | Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B47 | | Classification of International Standard | 65.140 | | Word Count Estimation | 5,524 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-06-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for determination of hydrogen peroxide in bee pollen enzymes. This standard applies to the determination of catalase had in bee pollen. | GB/T 23195-2008: Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for the determination of catalase in bee pollen. Ultaraviolet spectrophotometry
ICS 65.140
B47
National Standards of People's Republic of China
Determination of catalase bee pollen too
UV spectrophotometry
Posted 2008-12-31
2009-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is proposed and managed by China Federation of Supply and Marketing Cooperatives.
This standard was drafted. Guangzhou City Po Sang Park Co., Ltd., South China Agricultural University.
The main drafters of this standard. Long Zheng Yao, Liu Wei, Chen Weibin.
Determination of catalase bee pollen too
UV spectrophotometry
1 Scope
This standard specifies the method for determination of bee pollen of catalase.
This standard is applicable by measuring catalase in bee pollen.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Hydrogen peroxide absorbs strongly at a wavelength of 240nm, catalase can decompose hydrogen peroxide, the reaction solution in a certain range of absorbance
Inner circle with the reaction time reduced, can be determined according to the rate of change in absorbance measured catalase activity.
4 Reagents
Unless otherwise indicated in the analysis using only analytical reagent and in line with GB/T 6682 requirements distilled or deionized water.
4.1 phosphate buffer. pH 6.8 to 7.0, at a concentration of 50mmol/L, containing 1mmol/L disodium ethylenediamine tetraacetate (EDTA).
Weigh accurately 17.91gNa2HPO4 · 12H2O add distilled water and diluted to 500mL, as solution A;
Weigh accurately 7.80gNaH2PO4 · 2H2O add distilled water and diluted to 500mL, as solution B;
The exact amount of fluid and take 55.0mLA 45.0mLB liquid in 200mL beaker, 0.07gEDTA, add distilled water mixed
Even (added to about 150mL), calibrated to 6.8 to 7.0 with a pH meter, transferred to 200mL volumetric flask, dilute to volume, and mix.
4.2 hydrogen peroxide solution. volume fraction of 0.1%.
Imbibe 0.5mL30% hydrogen peroxide is placed 50mL brown volumetric flask, with phosphate buffer (4.1) to volume, mix,
Accurately taken from the triangular 10.0mL in 100mL Erlenmeyer flask (dark), and then accurately weighed 20.0mL phosphate buffer solution (4.1) was added to the mixed
Even that was.
4.3 liquid nitrogen.
5 Equipment
5.1 UV spectrophotometer.
5.2 refrigerated centrifuge.
5.3 constant temperature water bath.
5.4 pH meter.
5.5 Analysis of balance. a sense of the amount of 0.1mg.
5.6 pipette. 100μL, 5000μL.
6 Preparation of test solution
Weigh 1g sample to the nearest 1mg, has been placed with the right amount of liquid nitrogen ice-cold porcelain mortar while adding liquid nitrogen side grinding, the sample to be fully RESEARCH
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