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GB/T 22429-2008 English PDF

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GB/T 22429-2008: Rapid screening for salmonella, escherichia coli 0157 and listeria monocytogenes in foods -- Enzyme-linked immunoassay method
Status: Valid
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GB/T 22429-2008English169 Add to Cart 3 days [Need to translate] Rapid screening for salmonella, escherichia coli 0157 and listeria monocytogenes in foods -- Enzyme-linked immunoassay method Valid GB/T 22429-2008

PDF similar to GB/T 22429-2008


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Basic data

Standard ID GB/T 22429-2008 (GB/T22429-2008)
Description (Translated English) Rapid screening for salmonella, escherichia coli 0157 and listeria monocytogenes in foods -- Enzyme-linked immunoassay method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 7,758
Date of Issue 2008-10-19
Date of Implementation 2009-02-01
Quoted Standard GB/T 4789.4; GB/T 4789.6; GB/T 4789.28-2003; GB/T 4789.30
Regulation (derived from) National Standard Approval Announcement 2008 No.17 (Total No.130)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the Salmonella in food, verification step enterohemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria ELISA and judgment rule. This standard applies to all kinds of foods Salmonella, qualitative test enterohemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria.

GB/T 22429-2008: Rapid screening for salmonella, escherichia coli 0157 and listeria monocytogenes in foods -- Enzyme-linked immunoassay method


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Rapid screening for salmonella, escherichia coli 0157 and listeria monocytogenes in foods. Enzyme-linked immunoassay method ICS 07.100.30 C53 National Standards of People's Republic of China Salmonella in food, Enterohaemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria Rapid screening test ELISA Posted 2008-10-19 2009-02-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Standardization Technical Committee of Food Industry and focal points. This standard was drafted. Hangzhou Quality and Technical Supervision and Inspection Institute, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Food Drug Control. The main drafters of this standard. Xiao Hailong, Gu Ming, Rui Chang, Han Wei, Xu Weidong, Bao Ying, Wang Ying. Salmonella in food, Enterohaemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria Rapid screening test ELISA

1 Scope

This standard specifies the food Salmonella, enterohaemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria enzyme-linked Test procedures and determine the principles of immunization. This standard applies to all kinds of foods Salmonella, enterohaemorrhagic Escherichia coli O157 and Listeria monocytogenes bacteria Qualitative test.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 4789.4 Microbiological examination of food hygiene inspection salmonella GB/T 4789.6 Microbiological examination of food hygiene cause diarrhea Escherichia coli test GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents GB/T 4789.30 Microbiological examination of food hygiene Listeria monocytogenes bacteria test

3 Method summary

Samples for the enrichment process, enrichment broth after heat treatment moved coated within specific antibodies (antibody) solid container, with a target of anti-bacteria Combined, washed away unbound other ingredients; adding specific enzyme-labeled antibody (secondary antibody), washed other components unbound again; join a particular substrate React to form a colored compound or a fluorescent compound, by detecting the absorbance or fluorescence intensity, compared with the reference value, the test results obtained.

4 Equipment and Materials

4.1 enzyme immunoassay analyzer (VIDAS instrument or similar 1)). 1) VIDAS instrument is provided by the French Merieux product trade name. This information is given for the convenience of users of this standard does not mean to Approval of the product. If other product has the same effect, you can use these equivalent products. 4.2 Refrigerator. 2 ℃ ~ 8 ℃. 4.3 incubator. 30 ℃ ± 1 ℃, 36 ℃ ± 1 ℃, 42 ℃ ± 1 ℃. 4.4 homogenizer. 4.5 Electronic Balance. Range 0g ~ 500g, a sense of the amount of 0.1g. 4.6 vortex mixer. 4.7 constant temperature water bath. 4.8 sterilization equipment. 5 medium, reagents and kits 5.1 buffered peptone water (BP). according to the provisions of 4.12 GB/T 4789.28-2003 formulated.

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