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GB/T 22250-2025: Determination of chlorogenic acid in health foods
Status: Valid GB/T 22250: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 22250-2025 | English | 199 |
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Determination of chlorogenic acid in health foods
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GB/T 22250-2025
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| GB/T 22250-2008 | English | 139 |
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Determination of chlorogenic acid in health foods
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GB/T 22250-2008
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PDF similar to GB/T 22250-2025
Basic data | Standard ID | GB/T 22250-2025 (GB/T22250-2025) | | Description (Translated English) | Determination of chlorogenic acid in health foods | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X83 | | Classification of International Standard | 67.050 | | Word Count Estimation | 10,157 | | Date of Issue | 2025-03-28 | | Date of Implementation | 10/1/2025 | | Older Standard (superseded by this standard) | GB/T 22250-2008 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 22250-2025: Determination of chlorogenic acid in health foods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.050
CCSX83
National Standard of the People's Republic of China
Replace GB/T 22250-2008
Determination of Chlorogenic Acid in Health Food
Released on 2025-03-28
2025-10-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document specifies the technical requirements for food quality. For food safety requirements, please refer to relevant laws, regulations, policies and food safety standards.
document.
This document replaces GB/T 22250-2008 "Determination of chlorogenic acid in health foods".
In addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2008 edition);
b) The sample preparation and sample handling have been changed (see 7.1, 7.2, and 5.1 of the.2008 edition);
c) The chromatographic reference conditions have been changed (see 7.3, 5.3 of the.2008 edition);
d) The detection limit and quantification limit have been changed (see Chapter 10, Chapter 1 of the.2008 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee for Standardization of Special Foods (SAC/TC466).
This document was drafted by. China Light Industry Technology Innovation Center Co., Ltd., China Food Fermentation Industry Research Institute Co., Ltd., Shenzhen Metrology Quality Management Co., Ltd.
Quality Inspection Institute, China Customs Science and Technology Research Center, Perfect (Guangdong) Daily Necessities Co., Ltd., Guangzhou Dongpeng Food and Beverage Co., Ltd.
Co., Ltd., Tianjin University of Science and Technology, China Light Industry Inspection and Certification Co., Ltd., and Simao Customs Comprehensive Technical Service Center.
The main drafters of this document are. Wu Zhuying, Zhong Qiding, Zhang Zhaohui, Zhang Xieguang, Liu Ming, Li Junbo, Hu Hai'e, Lan Xiong, Zhang Cuiying, Liu Yang,
Wu Yifan, Chen Nannan, Guo Xinguang, Bie Wei, Wei Jianming, Li Xueli, Qi Zhengyou, Ji Xin, Chai Xiao, Zeng Lubin, and Zhang Xin.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2008 as GB/T 22250-2008;
---This is the first revision.
Determination of Chlorogenic Acid in Health Food
1 Scope
This document describes a method for the determination of chlorogenic acid in health foods by HPLC.
This document applies to chlorogenic acid in health foods in dosage forms such as tablets, hard candies, hard capsules, soft capsules, oral liquids, beverages, etc.
Determination.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
The chlorogenic acid in the sample was extracted with 70% methanol solution, separated by high performance liquid chromatography, detected by ultraviolet detector, and determined by retention time.
Quantitative analysis by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Reagents
5.1.1 Water. Grade 1 water as specified in GB/T 6682.
5.1.2 Acetonitrile (C2H3N). chromatographic grade.
5.1.3 Petroleum ether (boiling range 30℃~60℃).
5.2 Reagent preparation
5.2.1 70% (volume fraction) methanol solution. measure 700 mL of methanol (chromatographic grade), dilute to 1000 mL with water, and mix well.
5.2.2 0.1% (volume fraction) phosphoric acid solution. Pipette 1 mL of phosphoric acid, dilute to 1000 mL with water, and mix well.
5.3 Standard substances or standard samples
Chlorogenic acid (C16H18O9, CAS No.. 327-97-9). purity not less than 98%, or a nationally certified standard substance or
Standard samples.
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