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GB/T 21981-2008 English PDF

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GB/T 21981-2008: Determination of hormone multiresidues in foodstuffs of animal origin -- LC-MS/MS method
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PDF similar to GB/T 21981-2008


Standard similar to GB/T 21981-2008

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Basic data

Standard ID GB/T 21981-2008 (GB/T21981-2008)
Description (Translated English) Determination of hormone multiresidues in foodstuffs of animal origin -- LC-MS/MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.120
Word Count Estimation 30,350
Date of Issue 2008-06-06
Date of Implementation 2008-07-03
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.9 (Total No.122)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the hormone residues in food of animal origin amount of liquid chromatography-mass spectrometry/mass spectrometry. This standard applies to pork, liver, eggs, milk, beef, chicken and shrimp and other foods of animal origin in 50 kinds of hormone residues quantitative determination and confirmation.

GB/T 21981-2008: Determination of hormone multiresidues in foodstuffs of animal origin -- LC-MS/MS method


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Determination of hormone multiresidues in foodstuffs of animal origin. LC-MS/MS method ICS 67.120 X04 National Standards of People's Republic of China Animal Food hormone multi-residue methods Liquid chromatography - mass spectrometry/mass spectrometry Posted 2008-06-06 2008-07-03 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A and Appendix B for the normative appendix, Appendix C, Appendix D, Appendix E, Appendix F and Appendix G are informative appendices. This standard was proposed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard by the National Certification and Accreditation Administration of People's Republic of China. This standard was drafted. Beijing Center for Disease Control and Prevention, Chinese Center for Disease Control Nutrition and Food Safety, Chinese test Quarantine Science Research Institute. The main drafters of this standard. Shao Bing, Yang Yi, Zhang Jing, Wu Yongning, Chu Xiaogang. Animal Food hormone multi-residue methods Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard specifies the hormone residues in food of animal origin amount of liquid chromatography - mass spectrometry/mass spectrometry. This standard applies to meat, liver, eggs, milk, beef, chicken and shrimp and other foods of animal origin 50 kinds of hormones (see Appendix A) residues Quantitative determination and confirmation.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

A sample of the target compound homogenized, digested with methanol - aqueous extraction, purification by solid phase extraction and liquid chromatography - mass spectrometry/mass Spectrometer, internal standard.

4 Reagents and materials

Except where noted, all reagents used in this standard HPLC grade water as a water GB/T 6682 regulations. 4.1 methanol. 4.2 dichloromethane. 4.3 acetonitrile. 4.4 formic acid. 4.5 acetic acid. AR. 4.6 sodium acetate (NaAC · 4H2O). AR. 4.7 β- glucuronidase/aryl sulfatase solution (β-glucuronidase/arylsulfatase). 4.5U/mLβ- glucuronide Enzymes, 14U/mL aryl sulfatase. 4.8 acetic acid - sodium acetate buffer solution (pH5.2). Weigh 43.0g of sodium acetate (NaAC · 4H2O), was added 22mL of acetic acid with water-soluble Solutions and set the volume to 1000mL, adjusted to pH 5.2 with acetic acid. 4.9 methanol - water (1 + 1, volume ratio). Take 50mL of methanol and 50mL of water. 4.10 methylene chloride - methanol solution (7 + 3, volume ratio). Take 70mL of dichloromethane and methanol mixed 30mL. 4.11 0.1% formic acid. precise amount of formic acid 1mL diluted with water to 1000mL. 4.12 Standard. norepinephrine androstenedione, trenbolone, boldenone, fluoxymesterone, nandrolone, androstenedione, testosterone, DHEA Pula, methyltestosterone, testosterone iso Ketones, DHEA, stanozolol, 17β- hydroxy-androstan-3-one, the United States and testosterone, danazol, Tomio nandrolone, hydroxymethyl androstenedione, Methandriol, estradiol, three Alcohol, estrone, ethinyl estradiol, estrone hexane, diethylstilbestrol, dienestrol, norethindrone, 21α- hydroxy progesterone, 17α- hydroxy progesterone, levonorgestrel, Medroxyprogesterone acetate, megestrol acetate, progesterone, medroxyprogesterone acetate, chlormadinone, triamcinolone, aldosterone, prednisone, cortisone, hydrogen Cortisone, prednisolone, Flumetasone, dexamethasone, fludrocortisone acetate, methyl prednisolone, beclomethasone, triamcinolone acetonide, fluocinolone, Flumetholon, budesonide, clobetasol propionate, purity greater than 97%. English substance name and CAS number in Appendix A.

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