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GB/T 21533-2008: Determination of starch syrup in honey -- Ion chromatography
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21533-2008 | English | 319 |
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Determination of starch syrup in honey -- Ion chromatography
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GB/T 21533-2008
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Basic data | Standard ID | GB/T 21533-2008 (GB/T21533-2008) | | Description (Translated English) | Determination of starch syrup in honey -- Ion chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X31 | | Classification of International Standard | 67.180.10 | | Word Count Estimation | 8,885 | | Date of Issue | 2008-04-09 | | Date of Implementation | 2008-09-01 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.5 (Total No.118) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the honey fructose syrup, maltose syrup, isomalt syrup, determination of caramel syrup and other starch syrup. This standard applies to the determination of starch in honey syrup. This standard detection limit: 5 % starch syrup. | GB/T 21533-2008: Determination of starch syrup in honey -- Ion chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of starch srupy in honey. Ion chromatography
ICS 67.180.10
X31
National Standards of People's Republic of China
Honey starch syrup by Ion Chromatography
Posted 2008-04-09
2008-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
This standard was proposed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China.
The standard by the China Federation of Supply and Marketing Cooperatives.
This standard was drafted. Dalian City Product Quality Supervision, Inspection.
The main drafters of this standard. Pan Wei, Wang Chunyan, Zheng Shunli, Yu Lijun, Mou, Zhang, Li Peng, Dongguang Bin, Li Haiyan, Jiang Jun.
This standard is the first release.
Honey starch syrup by Ion Chromatography
1 Scope
This standard specifies the honey fructose syrup, maltose syrup, maltose syrup different measurement method, caramel syrup and other starch syrup.
This standard applies to the determination of starch in honey syrup.
The standard detection limit. 5% starch syrup.
Principle 2
Honey does not contain pentasaccharide (DP5) above oligosaccharide, and a variety starch syrup inclusive pentasaccharide (DP5) or more oligosaccharides, using a gel volume
Removal of sample exclusion of fructose, glucose, directly through the anion-exchange oligosaccharides enriched chromatography - electrochemical detector, the pentasaccharide
Presence (DP5) above is determined as an indicator of honey oligosaccharides in starch syrup.
3 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade, the water is 18.2ΜΩ/cm high water.
3.1 polyacrylamide gel microspheres, particle size 45μm ~ 90μm, fractionated molecular weight range of 100 to 1800, according to the use of said
SHEET hydrated and degassed.
NOTE. You can use Bio-Gel P-2Gel polyacrylamide gels or equivalent gel material?.
3.2 gel column. The polyacrylamide gel (3.1) wet mount 1.5cm × 15cm Empty Cartridges charged gel height
10cm, 1cm above the upper end to keep the water layer, to avoid drying.
3.3 column frame.
3.4 Maltose standard stock solution. Weigh chromatographically pure maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose
Each standard substance 10.0mg, were dissolved with water to 10mL, formulated at a concentration of 1mg/mL stock solution in a brown bottle 4 ℃
Under storage.
Maltose 3.5 standard mixed solution. draw a certain amount of maltose standard stock solution (3.4), according to Table 1 Standard mixing water formulated maltose
Use solution, stored at 4 ℃ not more than 30d. This solution was used for sample chromatogram oligosaccharides retention orientation in time.
Table 1 maltose formulated standard mixed solution
Standards maltose maltotriose maltotetraose maltopentaose maltohexaose maltoheptaose
The amount of stock solution to draw/mL 0.10 0.20 0.30 0.40 0.50 1.00
Volume Volume/mL 10
Mix concentration/(μg/mL) 10 20 30 40 50 100
3.6 stock solution of 50% sodium hydroxide. compliance with ion chromatography purity.
3.7 anhydrous sodium acetate. compliance with ion chromatography purity.
3.8 0.45μm membrane sample. water.
4 Instrument
4.1 Ion Chromatography. distribution of chemical detector.
4.2 Analysis of balance. a sense of the amount of 0.1mg.
5 Sample Preparation
5.1 Weigh 2.0g of mixture of honey as a sample, dissolved in water volume to 20mL, water-based membrane with 0.45μm filtered and the filtrate
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