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GB/T 21323-2007: Determination of aminoglycosides residues in animal tissues -- HPLC-MS/MS method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21323-2007 | English | 289 |
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Determination of aminoglycosides residues in animal tissues -- HPLC-MS/MS method
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GB/T 21323-2007
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Basic data | Standard ID | GB/T 21323-2007 (GB/T21323-2007) | | Description (Translated English) | Determination of aminoglycosides residues in animal tissues -- HPLC-MS/MS method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.120 | | Word Count Estimation | 11,111 | | Date of Issue | 2007-10-29 | | Date of Implementation | 2008-04-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | China National Standard Approval Announcement2007 No.13 (Total No.113) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the animal tissue spectinomycin, hygromycin B, dihydrostreptomycin, streptomycin, amikacin, kanamycin, apramycin, tobramycin, gentamicin neomycin and neomycin 10 kinds of aminoglycoside residues performance liquid chromatography tandem mass spectrometry and confirmation methods. This standard applies to animal offal, muscle and 10 kinds of aquatic products aminoglycoside residues measured. | GB/T 21323-2007: Determination of aminoglycosides residues in animal tissues -- HPLC-MS/MS method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of aminoglycosides residues in animal tissues. HPLC-MS/MS method
ICS 67.120
X04
National Standards of People's Republic of China
Determination of aminoglycoside residues in animal tissues
High performance liquid chromatography - mass spectrometry/mass spectrometry
Posted 2007-10-29
2008-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The Standard Appendix A, Appendix B is an informative annex.
This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China.
This standard was drafted. People's Republic of China Hunan Entry-Exit Inspection and Quarantine Shandong People's Republic of China
Bureau, Hunan Normal University, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Mr Wong, Lin Liming, Zhang Hongwei, Zhang Ying, Chen Bo, Peng Tao, Li Xiaojuan, Wang Meiling, Yan Hongfei.
Determination of aminoglycoside residues in animal tissues
High performance liquid chromatography - mass spectrometry/mass spectrometry
1 Scope
This standard specifies the animal tissues spectinomycin, hygromycin B, dihydrostreptomycin, streptomycin, amikacin, kanamycin, AMP
Vancomycin, tobramycin, gentamicin and neomycin aminoglycoside residues in 10 kinds of high performance liquid chromatography - tandem mass spectrometry and confirmation
method.
This standard applies to animal organs, muscles, and aquatic products in 10 kinds of aminoglycoside residues determination.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
Principle 3
Sample aminoglycoside drug residues, using phosphate buffer extracted through C18 solid-phase extraction (SPE), and concentrated using heptafluoro
Butyric acid as an ion pair reagent, high performance liquid chromatography - mass spectrometry/mass spectrometry, external standard.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 Methanol. HPLC grade.
4.2 glacial acetic acid. HPLC grade.
4.3 formic acid. HPLC grade.
4.4 heptafluoro acid. purity ≥99%.
4.5 concentrated hydrochloric acid.
4.6 sodium hydroxide.
4.7 TCA. Purity ≥99%.
4.8 disodium edetate (Na2EDTA). purity ≥99%.
4.9 potassium dihydrogen phosphate.
4.10 heptafluorobutyric acid solution (HFBA). 100mmol/L, were accurately taken 6.5mL heptafluorobutyric acid (4.4), dilute with water to 500mL
(4 ℃ in the dark for up to 6 months).
4.11 heptafluorobutyric acid solution. 20mmol/L, were accurately taken 100mmol/L heptafluorobutyric acid solution 50mL (4.10), diluted with water to
250mL (4 ℃ in the dark for up to 6 months).
4.12 phosphate buffer (containing 0.4mmol/LEDTA and 2% trichloroacetic acid solution). Weigh accurately potassium dihydrogen phosphate (4.9) 1.36g,
Dissolved in 980ml water, pH adjusted with hydrochloric acid 1.0mol/L to 4.0, were added Na2EDTA (4.8) 0.15g and TCA
(4.7) 20g, and mix to dissolve and dilute to 1000mL (4 ℃ can be stored in the dark for 1 month).
4.13 formic acid. 0.1% (volume fraction), Imbibe 1.0mL of formic acid (4.3) in 1000mL volumetric flask, dilute to the mark,
Mix well.
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