Search result: GB/T 15686-2008 (GB/T 15686-1995 Older version)
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 15686-2008 | English | 139 |
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Sorghum -- Determination of tannin content
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GB/T 15686-2008
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| GB/T 15686-1995 | English | 199 |
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Sorghum--Determination of tainnin content
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GB/T 15686-1995
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| Standard ID | GB/T 15686-2008 (GB/T15686-2008) | | Description (Translated English) | Sorghum. Determination of tannin content | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B20 | | Classification of International Standard | 67.040 | | Word Count Estimation | 6,640 | | Date of Issue | 2008-11-04 | | Date of Implementation | 2009-01-20 | | Older Standard (superseded by this standard) | GB/T 15686-1995 | | Quoted Standard | GB 5491; GB/T 21305 | | Adopted Standard | ISO 9648-1988, MOD | | Drafting Organization | Henan University of Technology | | Administrative Organization | National Standardization Technical Committee Agri | | Regulation (derived from) | National Standard Approval Announcement 2008 No.18 (Total No.131) | | Proposing organization | National Grain Bureau | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Administration Committee | | Summary | This standard provides a general method for the determination of tannin sorghum. This measurement method is not determined for certain types of polyphenols. Between the results of animal experiments with rooster sorghum measured metabolizable energy and determination of the method has good negative correlation, demonstrate the effectiveness of this method. |
GB/T 15686-2008
Sorghum. Determination of tannin content
ICS 67.040
B20
National Standards of People's Republic of China
Replacing GB/T 15686-1995
Determination of tannin content of sorghum
(ISO 9648. 1988, MOD)
Posted 2008-11-04
2009-01-20 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This revised standard adopts ISO 9648. 1988 "Determination of tannin content Sorghum" (in English).
This standard and ISO 9648. 1988 is a technical difference.
--- Since the original standard referenced standard "ISO 950. 1979 grain samples (grain)" has been abolished, it is changed to refer to "GB 5491
Grain, oil inspection for sampling, and sample method. "
For ease of use, this standard made the following editorial changes.
--- "This International Standard" be replaced by "this standard";
--- Remove the preface international standards, add new foreword;
--- The original text of the "reference standard" to "normative references" and add "undated reference standard, the latest version
This applies to this standard, "the text;
--- References "ISO 712. 1985 Cereals and measurement (routine reference method) moisture content cereal product" to quote
"GB/T 21305 Cereals and cereal products - Determination of moisture content of the General Law";
--- In order to more clearly articulate the tannin content of Chapter 9 of the formula Ba 2 × m1
100-H
Ba to X = 2 × m1
100-H
This standard replaces GB/T 15686-1995 "Determination of tannin content of sorghum."
This standard and GB/T 15686-1995 compared to the main changes are as follows.
--- Tannic acid standard using ISO 9648. 1988 have recommended Merck's 773 tannic acid;
The standard proposed by the National Food Authority.
This standard by the National Standardization Technical Committee OILS.
This standard was drafted. Henan University.
The main drafters of this standard. the right to Christine Hall, Fan Lu, Zhou Zhanming.
This standard replaces the standards previously issued as follows.
--- GB/T 15686-1995.
Determination of tannin content of sorghum
1 Scope
The standard provides a general method for the determination of tannin sorghum, this assay is not for a certain type of polyphenols
The test substance. Between the results of animal experiments with the rooster measured metabolizable energy of sorghum with the method of determination and has a good negative correlation,
Demonstrate the effectiveness of this method.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB 5491 grain and oilseeds testing for sampling, and sample method
GB/T 21305 Cereals and cereal products - Determination of moisture conventional method (GB/T 21305-2007, ISO 712.1998, IDT)
Principle 3
After the extraction of tannin sorghum with dimethyl formamide solution by centrifugation, the supernatant was added to a solution of ferric ammonium citrate and ammonia solution, color, water
Blank control, measured by a spectrophotometer absorbance at 525nm Department, with tannic acid standard curve measured sorghum tannin content.
4 Reagents
All reagents were of analytical grade, distilled water or water of equivalent purity.
4.1 2g/L tannic acid solution. The solution can be stored for a week.
Note. Due to different sources of tannic acid standard curve impact, therefore, recommend the use of Merck's 773 tannic acid (Merckreference773tannic
acid) 1) as a reference to facilitate comparison between laboratory measurement results.
1) Merck 773 tannic acid is a commercially available product, the user of this standard to provide such information, does not mean it has been approved ISO
The product.
4.2 8.0g/L ammonia (NH3) solution.
4.3 75% dimethyl formamide solution. Take-dimethylformamide 75mL solution in 100mL flask, diluted with water, after cooling add
Water to the mark.
Warning --- When dimethylformamide is inhaled or in contact with the skin, harmful to health, and eye irritation.
4.4 3.5g/L ferric ammonium citrate (iron content of 17% to 20%) solution. 24h preparation before use. Since the iron content of the citrate Movies
Sound measurement results, should pay special attention to its content.
5 Instruments
Conventional laboratory equipment and the following instruments.
5.1 mill. pulverizing the sample and all through the sieve (5.2).
5.2 sieve. aperture of 0.5mm.
5.4 tube. capacity of about 50mL, with closures.
5.5 reciprocating mechanical stirrer or magnetic stirrer.
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