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US$149.00 · In stock Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 14926.58-2008: Laboratory animal -- Method for examination of infectious canine hepatitis virus Status: Valid GB/T 14926.58: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14926.58-2008 | English | 149 |
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Laboratory animal -- Method for examination of infectious canine hepatitis virus
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GB/T 14926.58-2008
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| GB/T 14926.58-2001 | English | 199 |
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Laboratory animal -- Method for examination of infectious caninc hepatitis virus (ICHV)
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GB/T 14926.58-2001
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PDF similar to GB/T 14926.58-2008
Basic data | Standard ID | GB/T 14926.58-2008 (GB/T14926.58-2008) | | Description (Translated English) | Laboratory animal -- Method for examination of infectious canine hepatitis virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B44 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 5,574 | | Date of Issue | 2008-12-03 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 14926.58-2001 | | Quoted Standard | GB/T 14926.50; GB/T 14926.54 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.19 (Total No.132) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the infectious canine hepatitis virus (ICHV) detection methods, reagents. This section applies to dogs ICHV detection. |
GB/T 14926.58-2008: Laboratory animal -- Method for examination of infectious canine hepatitis virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Method for examination of infectious canine hepatitis virus
ICS 65.020.30
B44
National Standards of People's Republic of China
Replacing GB/T 14926.58-2001
Laboratory Animal Infectious Canine Hepatitis Virus Detection
Posted 2008-12-03
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods.
Since the implementation of this section instead of GB/T 14926.58-2001 "animal infectious canine hepatitis virus detection methods."
This part of the main technical differences compared with the GB/T 14926.58-2001 as follows.
a) Infectious canine hepatitis is one of the major deadly infectious diseases in dogs, widely popular in our dog population is the foundation level SPF dogs and dogs
Will review the project;
b) "6 results found" added "immune antibody level pass rate of 6.3 based dog groups. the passing rate of antibody = (subject animals
Antibody-positive/is the total number of seized animals) × 100%. Ground-level pass rate of antibody dog \u200b\u200bpopulation greater than or equal than 70% penalty
For the dog population qualified immunity "content.
This part of the National Standardization Technical Committee of experimental animals and focal points.
This part of the National Standardization Technical Committee responsible for drafting the experimental animals.
The main drafters of this section. TIAN Ke-gong, He contention, Fan Wei.
This part of the standard replaces the previous editions are.
--- GB/T 14926.58-2001.
Laboratory Animal Infectious Canine Hepatitis Virus Detection
1 Scope
GB/T 14926 provisions of this part of the infectious canine hepatitis virus (ICHV) detection methods, reagents and the like.
This section applies to dogs ICHV detection.
2 Normative references
The following documents contain provisions which, through reference GB/T 14926 in this section constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
ELISA GB/T 14926.50 Experimental Animals
GB/T 14926.54 animal hemagglutination inhibition test
Principle 3
According to the principles of immunology, antigen detection using ICHV ICHV dog serum antibody; or under ICHV under certain conditions, to pour
Set person "O" type red blood cells, this ability agglutination of red blood cells can be inhibited by specific antibodies principles detection canine serum ICHV antibody.
4 Reagents and equipment
4.1 Reagents
4.1.1 ELISA antigen
4.1.1.1 Specific Antigen
MDCK cells were infected with ICHV, 3d ~ 4d after inoculation, when the lesion reached +++ ~ ++++ (- negative; + Light; + in;
+++ Weight; ++++ serious, when the same below) harvest. After three freeze-thaw or sonication, cell debris was removed by low speed centrifugation, supernatant was
After ultracentrifugation concentrated into ELISA antigen.
4.1.1.2 normal antigen
After freezing and thawing broken by low-speed centrifugation to remove cell debris and the supernatant obtained by MDCK cells.
4.1.2 hemagglutinin
ICHV inoculated MDCK cells, cultured 3d ~ 4d, when the disease reaches +++ ~ ++++ cultures were harvested. Freezing and thawing three times or
After sonication, cell debris was removed by low speed centrifugation, the supernatant aliquoted after cryopreservation.
4.1.3 positive serum
ICHV experimentally infected with canine serum or natural infection.
4.1.4 negative serum
No ICHV infection without immune dog serum.
4.1.5 conjugate
Horseradish peroxidase-labeled goat anti-dog or rabbit IgG antibody.
4.1.6 people "O" type red blood cells.
4.2 Equipment
4.2.1 microplate reader.
4.2.2 37 ℃ incubator or water bath.
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