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GB/T 14926.10-2008: Laboratory animal -- Method for examination of tyzzerorganism
Status: Valid GB/T 14926.10: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14926.10-2008 | English | 139 |
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Laboratory animal -- Method for examination of tyzzerorganism
| Valid |
GB/T 14926.10-2008
|
| GB/T 14926.10-2001 | English | 199 |
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Laboratory animal -- Method for examination of Tyzzer organism
| Obsolete |
GB/T 14926.10-2001
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| GB/T 14926.10-1994 | English | 199 |
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Method for examination of Bacillus piliformis in laboratory animal
| Obsolete |
GB/T 14926.10-1994
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PDF similar to GB/T 14926.10-2008
Basic data | Standard ID | GB/T 14926.10-2008 (GB/T14926.10-2008) | | Description (Translated English) | Laboratory animal -- Method for examination of tyzzerorganism | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B44 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 6,649 | | Date of Issue | 2008-12-03 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 14926.10-2001 | | Quoted Standard | GB/T 14926.50; GB/T 14926.52 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.19 (Total No.132) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies a method for detecting pathogens Taize experimental animals. This section applies to mice, hamster and rabbit Taize pathogen detection, rats, guinea pig. | GB/T 14926.10-2008: Laboratory animal -- Method for examination of tyzzerorganism---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Method for examination of tyzzer's organism
ICS 65.020.30
B44
National Standards of People's Republic of China
Replacing GB/T 14926.10-2001
Taize animal pathogen detection method
Posted 2008-12-03
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods.
Since the implementation of this section instead of GB/T 14926.10-2001 "Taize animal pathogen detection."
This part of the main technical differences compared with the GB/T 14926.10-2001 as follows.
a) Remove the cortisone stimulation test;
b) modify two serological methods.
This part of the National Standardization Technical Committee of experimental animals and focal points.
This section drafted by. National Standardization Technical Committee of experimental animals.
The main drafters of this section. Li Hong.
This part of the standard replaces the previous editions are.
--- GB/T 14926.10-1994, GB/T 14926.10-2001.
Taize animal pathogen detection method
1 Scope
GB/T 14926 provisions of this part of the detection method Taize animal pathogens.
This section applies to mice, rats, guinea pigs, hamsters and rabbits Taize pathogen detection.
2 Normative references
The following documents contain provisions which, through reference GB/T 14926 in this section constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
ELISA GB/T 14926.50 Experimental Animals
GB/T 14926.52 animals immunofluorescence test
Principle 3
Animals infected Tyzzer serum can produce antibodies. Based on the principles of immunology, antigen detection using small Tyzzer
Mouse, rat, guinea pig, hamster and rabbit sera corresponding antibodies.
4 major equipment and materials
4.1 ordinary incubator.
4.2 Fluorescence microscopy.
4.3 biological microscope.
4.4 microplate reader.
4.5 tissue grinder.
4.6 polystyrene plates, 40 holes, 55 holes or 96 holes (removable or can not be removed), washed before drying, UV irradiation 1h.
4.7 micro pipette, capacity 5μL ~ 50μL, 50μL ~ 200μL.
4.8 ~ 10 printed with apertures 40 slides.
4.9 coverslip.
4.10 ultra-low temperature freezer or liquid nitrogen tank.
5 medium and reagents
5.1 ELISA antigen
5.1.1 Taize pathogen antigen
Taize infected by the pathogen, animals with severe liver necrosis of 1.10 after joining PBS tissue grinder system in an ice bath
Homogenized, 3600r/min centrifugal supernatant as antigen.
5.1.2 Negative control antigen
Taize unaffected pathogens of animal liver, made by the method 5.1.1.
5.2 antigen sheet
5.2.1 Antigen
Taize subject infected with a pathogen, an animal with severe hepatic tissue necrosis 1.10 with PBS and homogenized in an ice bath to adjust the concentration
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