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GB 28305-2012 English PDF

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GB 28305-2012: National food safety standards -- Food additives -- Potassium lactate
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB 28305-2012	English	229	Add to Cart	3 days [Need to translate]	National food safety standards -- Food additives -- Potassium lactate	Valid	GB 28305-2012

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Basic data

Standard ID	GB 28305-2012 (GB28305-2012)
Description (Translated English)	National food safety standards -- Food additives -- Potassium lactate
Sector / Industry	National Standard
Classification of Chinese Standard	C54;X40
Classification of International Standard	67.220.20
Word Count Estimation	10,127
Regulation (derived from)	Ministry of Health Bulletin 2012 No. 7
Issuing agency(ies)	Ministry of Health of the People's Republic of China
Summary	This Chinese standard applies to the production of lactic acid by the fermentation method and potassium hydroxide food additives made ??of potassium lactate.

GB 28305-2012: National food safety standards -- Food additives -- Potassium lactate

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National food safety standards.Food additives .Potassium lactate National Standards of People's Republic of China National standards for food safety Food additives potassium lactate 2012-04-25 release 2012-06-25 Implementation Issued by the Ministry of Health of the People's Republic of China National standards for food safety Food additives potassium lactate

1 Scope

This standard applies to the production of lactic acid and potassium hydroxide produced by the reaction of food additives made of potassium lactate. 2 chemical name, structural formula, molecular formula, relative molecular mass 2.1 Chemical name Potassium 2-hydroxypropionate 2.2 Structural formula 2.3 Molecular formula C3H5KO3 2.4 Relative molecular mass 128.17 (according to the.2007 international relative atomic weight)

3 technical requirements

3.1 sensory requirements. should be consistent with the provisions of Table 1. Table 1 sensory requirements The project requires a test method Colorless or yellowish color Odorless or characteristic odor State micro viscous clear liquid Take appropriate sample in a clean, dry glass beaker, In the natural light, observe the color and state, and smell its taste 3.2 Physical and chemical indicators. should be consistent with the provisions of Table 2. Table 2 Physical and chemical indicators Item Index Test Method Potassium lactate content, w /% ≥ 50.0a Appendix A, A.3 Color/(Black) ≤ 100 GB/T 605 pH 5.0 to 9.0 Appendix A, A.4 Chloride passed the test Appendix A, A.5 Heavy metals (Pb)/(mg/kg) ≤ 20 GB/T 5009.74 Lead (Pb)/(mg/kg) ≤ 2 GB 5009.12 Sulfate through Test Appendix A, A.6 Sodium, w /% ≤ 0.1 GB/T 5009.91 Cyanide through Test Appendix A, A.7 Methanol and methyl ester were tested in Appendix A, A.8 Citrate, oxalate, phosphate or tartrate through Test Appendix A, A.9 The reducing sugar is passed through Test Appendix A. A.10 a The actual detection value is 98.0% ~ 102.0% of the label identification value.

Appendix A

Testing method A.1 General provisions The reagents and water used in this standard, when not specified in other requirements, refer to the analysis of pure reagents and GB/T 6682-2008 in the provisions of the three Water level. Standard titration solution used in the test, the standard solution for the determination of impurities, preparations and products, without any other requirements, GB/T 601, GB/T 602, GB/T 603. The solution used in the test refers to water when it is not specified with the formulation of the solvent Solution. A.2 Identification test A.2.1 Reagents and materials A.2.1.1 hydrochloric acid solution. 0.1mol/L. A.2.1.2 sulfuric acid solution. 1mL sulfuric acid and 19mL water prepared. A.2.1.3 potassium permanganate test solution. 0.1mol/L. A.2.1.4 Morpholine solution. 1 4. A.2.1.5 Sodium nitrite ferricyanide solution. 50 g/L. A.2.2 Analysis steps A.2.2.1 identification of potassium. take platinum wire, with hydrochloric acid solution wet, dipped in the sample, in the colorless flame burning, the flame that was purple; but there are A small amount of sodium salt mixed, must be separated from the blue glass perspective can be identified. A.2.2.2 identification of lactate. take 5mL sample, add 2mL sulfuric acid solution mix, plus 2mL potassium permanganate test solution, heating, should There is acetal gas generated. Acetaldehyde gas identification using an equal volume of morphine solution and nitrosyl ferricyanide test solution mixture wet Filter paper, filter paper and gas contact with blue. A.3 Determination of potassium lactate content A.3.1 Reagents and materials A.3.1.1 Acetic anhydride. A.3.1.2 Glacial acetic acid. A.3.1.3 Standard titration solution for perchloric acid. c (HClO4) = 0.1 mol/L. A.3.1.4 Crystal violet indicator solution. 5g/L. A.3.2 Analysis steps A.3.2.1 Potentiometric titration Weigh the equivalent of about 300mg of potassium lactate liquid sample, accurate to 0.0001g, add 10mL acetic anhydride and 50mL ice Acetic acid, mixed evenly, put it aside for about 20min, with potentiometric titration to titration with perchloric acid standard titration, the same method to do blank titration. A.3.2.2 Manual titration method Weigh the equivalent of about 300mg of potassium lactate liquid sample, accurate to 0.0001g, add 10mL acetic anhydride and 50mL ice Acetic acid, mixed evenly, put it aside for about 20min, add 1 drop of crystal violet indicator solution, titration with perchloric acid standard titration, titration to the solution was blue Color, 30s does not fade, that is, the end of the titration, while the blank test to correct the results of titration. A.3.3 Calculation of results The potassium lactate content is expressed as the mass fraction w1 of potassium lactate (C3H5KO3), expressed in%, calculated according to the formula (A.1) w1 = (V1-V0) x c x 0.1282 m x 100% (A.1) Where. V1 --- sample titration consumption perchloric acid standard titration solution volume value in milliliters (mL); V0 --- blank titration consumption perchloric acid standard titration solution volume value in milliliters (mL); c - the actual concentration of perchloric acid standard titration solution in moles per liter (mol/L); 0.1282 --- The molar mass of potassium lactate (C3H5KO3) in grams per mole (g/mmol) [M (C3H5KO3) = 128.2]; m - the mass of the sample, in grams (g). The experimental results are based on the arithmetic mean of the parallel measurement results. The absolute difference between the parallel determination results shall not exceed 0.5%. A.4 Determination of pH Take 1 part of the sample, add 5 copies of equal volume of water, prepared into the test solution (15), shake and then measured with a pH meter. A.5 Determination of chloride A.5.1 Reagents and materials A.5.1.1 Chloride standard solution. 0.1 mg/mL. When used, the standard solution was diluted 10 times to a chloride (Cl) concentration of 0.01 mg/mL. A.5.1.2 Nitric acid solution. 1 2. A.5.1.3 Silver nitrate solution. 17 g/L. A.5.2 Analysis steps Weigh about 1g sample, accurate to 0.01g, dissolved in water, transferred to 50mL volumetric flask, add water to the volume, shake. quasi- Really take 5mL of this sample solution, placed in 50mL colorimetric tube, add water to about 35mL, add 10mL nitric acid solution, plus 1mL nitric acid Silver solution, diluted with water to 50mL, shake, placed in the dark 5min, placed on a black background, compared with the standard tube, the turbidity should not be deep Standard tube, that is, through the test. Standard tube preparation. accurate extraction of 5mL diluted 10 times after the chloride standard solution and sample tube at the same time the same treatment. A.6 Determination of Sulfate A.6.1 Reagents and materials A.6.1.1 hydrochloric acid solution. 3mol/L. A.6.1.2 barium chloride solution. 250 g/L. A.6.1.3 Sulfate standard solution. 0.1 mg/mL. A.6.2 Analysis steps Weigh 2g sample, accurate to 0.01g, placed in 50mL colorimetric tube, add appropriate amount of water, add hydrochloric acid to make neutral, continue to add water diluted to About 40mL, add 2mL hydrochloric acid solution, shake, add 5mL barium chloride solution, diluted with water to 50mL, shake well, place 10min, set On a black background, compared with the standard tube, the turbidity should not be deeper than the standard tube, that is, through the test. Standard tube preparation. Accurately absorb 1mL sulfate standard solution and sample tube at the same time the same treatment. Determination of cyanide Note. Potassium cyanide is highly toxic. This test should be carried out carefully in a fume hood to prevent skin contact, inhalation of solution and steam. A.7.1 Reagents and materials A.7.1.1 p-phenylenediamine hydrochloride. A.7.1.2 Pyridine. A.7.1.3 Hydrochloric acid. A.7.1.4 Potassium cyanide. A.7.1.5 n-butanol. A.7.1.6 Sodium hydroxide solution. 0.1 mol/L. A.7.1.7 Sodium hydroxide solution. 20 g/L. A.7.1.8 Phosphoric acid solution. 5 g/L. A.7.1.9 sodium arsenite solution. 20 g/L. A.7.1.10 bromine reagent. 100g/L, weighed 10g bromine, set with Vaseline coated glass bottle, add water shake shake to volume to 100mL. The liquid should be kept in dark places. A.7.2 Instruments and equipment Spectrophotometer. A.7.3 Analysis steps A.7.3.1 Preparation of Solution A. Weigh 0.2 g of p-phenylenediamine hydrochloride, add 100 mL of water, heat to dissolve, after cooling solid precipitation, the supernatant In the preparation of solution B. A.7.3.2 Preparation of Solution B Measure the amount of 128mL pyridine, add 365mL water, add 10mL hydrochloric acid, mix. Add 30 mL of Solution A to the preparation process Clear solution, mixed evenly, placed 24h after the reserve. The mixed solution was stored in a brown bottle for 3 weeks. A.7.3.3 Preparation of standard solutions Weigh 0.25g potassium cyanide, dissolved in 0.1mol/L sodium hydroxide solution, into the 100mL volumetric flask, add 0.1mol/L hydrogen Sodium oxide solution diluted to the mark, mix. Accurately measure the above 10mL of liquid, into the 1000mL volumetric flask, with 0.1mol/L hydrogen Sodium oxide solution diluted to the mark, mix. The cyanide concentration was 0.01 mg/mL. A.7.3.4 Preparation of sample solution Weigh the equivalent of 20.0g of potassium lactate containing a liquid sample, placed in 100mL volumetric flask, diluted with water to the mark, mix. A.7.3.5 Determination Accurately draw a 10 mL sample solution and place in a 50 mL beaker. Accurately draw 0.10 mL of standard solution and place it in another 50 mL burn Cup, add 10 mL of water. Place the two beakers in an ice bath and slowly add 20 g/L sodium hydroxide solution to adjust the pH of the solution at 9 to 10 Slow agitation to avoid overheating. Standing for 3min, and then slowly add phosphoric acid solution to adjust the pH of the solution in the 5 to 6, the adjustment process with a pH meter measuring. The solution was transferred to a 100 mL standard separatory funnel containing 25 mL of cold water, and the pH meter was rinsed with a small amount of cold water Cups, lotions are combined into their respective separatory funnels. Add 2mL bromine reagent, respectively, plug, mix. Respectively, add 2mL sodium arsenite solution, plug Tight, mix well. Add 10mL n-butanol, plug, mix. Finally add 5mL solution B, mix, place 15min. Separating the aqueous phase, The alcohol phase was obtained and filtered into a 1 cm cuvette. The absorbance of the two solutions was measured at 480 nm using a spectrophotometer. A.7.4 Determination of results The sample solution corresponding to the absorbance value is not greater than the standard solution corresponding to the absorbance value, that is, through the test. A.8 Determination of methanol and methyl esters A.8.1 Reagents and materials A.8.1.1 Potassium permanganate. A.8.1.2 Phosphoric acid. A.8.1.3 Sulfuric acid. A.8.1.4 oxalic acid. A.8.1.5 anhydrous methanol. A.8.1.6 Ethanol solution. 1 10. A.8.1.7 Potassium hydroxide solution. 5 mol/L. A.8.1.8 Alkaline Magenta - Sulfite Test Solution. A.8.2 Instruments and equipment Spectrophotometer. A.8.3 Analysis steps A.8.3.1 Preparation of Solution A. Weigh 3g potassium permanganate, dissolved in 15mL phosphoric acid and 70mL water mixed solution, diluted with water to 100mL. A.8.3.2 Preparation of Solution B Measure 50mL sulfuric acid, carefully added to 50mL of water, mix. After cooling, add 5g oxalic acid, dissolve and mix well. A.8.3.3 Preparation of standard solutions Weigh 1.0g of anhydrous methanol, into the 100mL volumetric flask, diluted with water to the mark, mix. Accurately absorb 1mL of this solution, move Into the 100mL volumetric flask, diluted with ethanol solution to the mark, mix. A.8.3.4 Preparation of sample solution 40.0 g of potassium lactate was weighed, placed in a round bottom flask, and 10 mL of water was added. Carefully add 30 mL of potassium hydroxide solution and connect The condenser, the water vapor was heated and distilled, the fractions were collected into a collection bottle which had been placed in 10 mL of ethanol and the fractions were collected until the collection About 80mL, stop. The fraction was diluted with water to 100.0 mL to give a sample solution. A.8.3.5 Determination Respectively, the amount of 10.0mL standard solution and sample solution, were placed in 25mL volumetric flask, respectively, by adding 5.0mL solution A, mixed uniform. 15min, respectively, by adding 2.0mL solution B, with a glass rod to stir the solution to colorless, add 5.0mL alkaline red - sulfite test Liquid, diluted with water to the mark, mix. 2h, respectively, the two solutions were placed in 1cm cuvette, with water as a blank control, with the light The photometer measures the absorbance of the two solutions at the maximum absorption wavelength near 575 nm. A.8.4 Determination of results The sample solution corresponding to the absorbance value is not greater than the standard solution corresponding to the absorbance value, that is, through the test. A.9 Determination of citrate, oxalate, phosphate or tartrate A.9.1 Reagents and materials A.9.1.1 Ammonia solution. 1 1. A.9.1.2 Hydrochloric acid solution. 1 5. A.9.1.3 calcium chloride solution. 75g/L, weighed 7.5g calcium chloride (CaCl2 · 2H2O), dissolved in water, diluted volume to 100mL. A.9.2 Analysis steps 5 mL of the sample was taken and diluted to 50 mL with just boiled and cooled water. Take 4 mL of diluted solution, with aqueous ammonia or salt Acid solution to adjust the sample pH of 7.3 to 7.7, add 1mL of calcium chloride solution in boiling water bath after heating 5min, the solution shall not produce muddy Turbidity, that is, through the test. A.10 Determination of reducing sugar In 10 mL of hot Felix solution, add 5 drops of the sample, should be no red precipitate, that is, through the test.

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