|
US$209.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 25543-2010: National food safety standards of food additives L-alanine
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB 25543-2010 | English | 209 |
Add to Cart
|
3 days [Need to translate]
|
National food safety standards of food additives L-alanine
| Valid |
GB 25543-2010
|
PDF similar to GB 25543-2010
Basic data | Standard ID | GB 25543-2010 (GB25543-2010) | | Description (Translated English) | National food safety standards of food additives L-alanine | | Sector / Industry | National Standard | | Classification of Chinese Standard | X42 | | Classification of International Standard | 67.220.20 | | Word Count Estimation | 9,989 | | Date of Issue | 2010-12-21 | | Date of Implementation | 2011-02-21 | | Regulation (derived from) | Ministry of Health Bulletin No. 19 of 2010 | | Issuing agency(ies) | Ministry of Health of the People's Republic of China | | Summary | This Chinese standard applies to L- aspartic acid as the raw material, obtained by enzymatic production of food additives L-alanine. | GB 25543-2010: National food safety standards of food additives L-alanine---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National food safety standards of food additives L-alanine
National Standards of People's Republic of China
National standards for food safety
Food Additives L-Alanine
2010-12-21 release
2011-02-21 Implementation
Issued by the Ministry of Health of the People's Republic of China
Foreword
Appendix A of this standard is a normative appendix.
National standards for food safety
Food Additives L-Alanine
1 Scope
This standard applies to L-aspartic acid as raw material, the enzymatic production of food additives L-alanine.
2 normative reference documents
The documents cited in this standard are indispensable for the application of this standard. Any reference to the date of the document, only the date of the edition
This applies to this standard. For undated references, the latest edition (including all amendments) applies to this standard.
3 molecular formula, structural formula and relative molecular mass
3.1 Molecular formula
C3H7NO2
3.2 Structural formula
3.3 Relative molecular mass
89.09 (according to the.2007 international relative atomic mass)
4 technical requirements
4.1 sensory requirements. should be consistent with the provisions of Table 1.
Table 1 sensory requirements
The project requires a test method
Color white
Organizational state Crystallization or crystalline powder
Take appropriate laboratory samples, placed in a clean, dry white porcelain dish, in the natural light, visual
Observe, smell the smell
4.2 Physical and chemical indicators. should be consistent with the provisions of Table 2.
Table 2 Physical and chemical indicators
Item Index Test Method
L-alanine (on a dry basis), w /% 98.5 to 101.5 Appendix A, A.4
Dry reduction, w /% ≤ 0.20 Appendix A, A.5
pH (50g/L aqueous solution) 5.7 to 6.7 Appendix A, A.6
Arsenic (As)/(mg/kg) ≤ 1 A.7 in Appendix A.
Heavy metals (Pb)/(mg/kg) ≤ 10 Appendix A A.8
Residue on ignition, w /% ≤ 0.20 Appendix A, A.9
Specific rotation αm (20 ° C, D)/[(°) · dm2 · kg-1] 13.5 to 15.5 Appendix A A.10
Appendix A
(Normative appendix)
Testing method
A.1 Warning
Some of the test procedures specified in the test method may result in a hazardous situation. Operators should take appropriate safety and protective measures.
A.2 General provisions
Unless otherwise stated, only the reagents identified as analytical reagents and the tertiary water specified in GB/T 6682-2008 are used in the analysis.
Standard titration solution used in the test method, standard solution for the determination of impurities, preparations and articles, in the absence of other requirements,
GB/T 601, GB/T 602 and GB/T 603.
A.3 Identification test
A.3.1 Reagents and materials
A.3.1.1 Potassium permanganate.
A.3.1.2 Indanetone solution. 20 g/L. Weigh 20.0 g of indanetetone, dissolved in water and diluted to 1000 mL.
A.3.1.3 Sulfuric acid solution. 1 30.
A.3.2 Analysis steps
A.3.2.1 Indanone test
Weigh about 1g of laboratory samples, accurate to 0.1g, dissolved in 1000mL water, take this solution 5mL, add 1mL indan three ketone dissolved
Liquid, heated to boiling, about 3min after the purple.
A.3.2.2 Oxidation test
Weigh about 0.2g of laboratory samples, dissolved in 10mL sulfuric acid solution, add 0.1g potassium permanganate, boil, have a strong stimulating odor B
Aldehyde production.
A.4 Determination of L-alanine content
A.4.1 Methodological Summary
Samples to formic acid as a co-solvent, glacial acetic acid as a solvent to crystal violet as an indicator, titration with perchloric acid standard titration, according to high consumption
The volume of L-alanine was calculated by the volume of the standard titration solution of chloric acid.
A.4.2 Reagents and materials
A.4.2.1 Glacial acetic acid.
A.4.2.2 anhydrous formic acid.
A.4.2.3 Perchloric acid standard titration solution. c (HClO4) = 0.1 mol/L.
A.4.2.4 Crystal violet indicator solution. 2g/L.
A.4.3 Analysis steps
A.4.3.1 Weigh dry matter A in about 0.2gA.5, accurate to 0.0001g, placed in 250mL dry Erlenmeyer flask, add 3mL
Anhydrous formic acid dissolved, add 50mL glacial acetic acid, add 2 drops of crystal violet indicator solution, titration with perchloric acid titration solution to the solution from blue to
Blue and green for the end.
A.4.3.2 At the same time as the determination, the same amount of reagent solution was used as a blank test in the same procedure as for the measurement.
A.4.4 Calculation of results
L-alanine (C3H7NO2) mass fraction w1, the value in%, calculated according to formula (A.1).
w1 =
(V1-V2) × c × M
m x 1000 x 100%
(A.1)
Where.
V1 --- sample consumption perchloric acid standard titration solution (A.4.2.3) volume in milliliters (mL);
V2 --- blank consumption Perchloric acid standard titration solution (A.4.2.3) volume in milliliters (mL);
c - the exact value of the perchloric acid standard titration solution concentration in moles per liter (mol/L);
m - the mass of the sample, in grams (g);
M - L-alanine in grams per mole (g/mol) (M = 89.09).
The arithmetic mean of the results of two parallel measurements is the result of the report. The absolute difference between the two parallel determinations is not more than 0.3%.
A.5 Determination of dry reduction
According to GB/T 6284. Determination, weighed 1g ~ 2g laboratory samples, accurate to 0.0001g. Take two parallel measurements
The arithmetic mean is the result of the measurement, and the absolute difference between the two parallel determinations is not more than 0.03%. Keep some dry matter (this is dry
A) was used for the determination of L-alanine content.
A.6 Determination of pH
According to GB/T 9724. At the time of measurement, approximately 5 g of laboratory samples were weighed, accurate to 0.01 g, plus about 20 mL of water without carbon dioxide
Dissolved and diluted to 100mL after the determination.
A.7 Determination of arsenic
According to GB/T 5009.76 arsenic spot method. Measure about 1g of laboratory samples, accurate to 0.01g. Limited amount of standard solution
System. with a pipette to remove 1.00mL arsenic limited standard solution (containing arsenic 0.001mg), the same sample with the same treatment.
A.8 Determination of heavy metals
A.8.1 Reagents and materials
A.8.1.1 thioacetamide solution. Weigh thioacetamide about 4g, accurate to 0.1g, dissolved in 100mL water, placed in the refrigerator.
Before the application, 1.0 mL of the solution was added to a mixture of 15 mL of sodium hydroxide solution (40 g/L), 50 mL of water and 20 mL of glycerin
5mL, placed in a water bath for 20s, cooling immediately use.
A.8.1.2 No carbon dioxide water.
A.8.1.3 Ammonium acetate buffer solution, pH = 3.5. Weigh 25.0 g of ammonium acetate, dissolved in 25 mL of water, plus 45 mL of 6 mol/L salt
Acid, adjusted to pH = 3.5 with dilute hydrochloric acid or dilute aqueous ammonia, and diluted with water to 100 mL.
A.8.1.4 Lead (Pb) standard solution. 1 μg/mL. This solution was prepared before use.
A.8.2 Analysis steps
Weigh about 10g of laboratory samples, accurate to 0.01g, with about 60mL without carbon dioxide water dissolved and diluted to 100mL, as the sample
Solution. Absorb the sample solution 12mL, placed in 25mL with a colorimetric tube, that is, A tube. Draw 10 mL of lead standard solution and 2 mL
The sample solution was placed in a 25 mL stoppered colorimetric tube and shaken well as a B tube (standard). Take 10 mL of carbon dioxide-free water and 2 mL of sample
Solution set 25mL with a colorimetric tube, shake, that is C tube (blank). In the A, B, C tube, each add 2mL ammonium acetate buffer solution,
Shake, respectively, add 1.2mL thioacetamide solution, stir and mix quickly.
Relative to the C tube, B tube showed a light brown. 2min, A tube color should not be deeper than the B tube.
A.9 Determination of burning residue
A.9.1 Reagents and materials
A.9.1.1 Sulfuric acid.
A.9.1.2 Sulfuric acid solution. 1 8.
A.9.2 Analysis steps
Weigh about 2g ~ 3g of laboratory samples, accurate to 0.0001g, placed at 800 ℃ ± 25 ℃ burning to the quality of the porcelain crucible
, Add the appropriate amount of sulfuric acid solution to completely wet the sample. Heat with a warm fire to the sample completely carbonized and cooled. Add about 0.5mL sulfuric acid dip
Wet residue, heated to sulfuric acid vapor by the above method. In the 800 ℃ ± 25 ℃ burning 45min. Into the dryer to cool to room temperature,
Weighing.
A.9.3 Calculation of results
The mass fraction w2 of the ignition residue, expressed in%, is calculated according to the formula (A.2)
w2 = m1m × 100%
(A.2)
Where.
m - the mass of the sample, in grams (g);
m1 - the mass of the residue, in grams (g).
The arithmetic mean of the results of two parallel measurements is the result of the report. The absolute difference between the two parallel determinations is not more than 0.02%.
A.10 Determination of specific rotation
A.10.1 Weigh 10g of laboratory samples, accurate to 0.0001g, add hydrochloric acid solution (1 1) dissolved, transferred to 100mL volumetric flask and
Diluted with hydrochloric acid solution (1 1) to the scale, shake.
The value of the specific rotation αm (20 ° C, D) is expressed by (°) · dm2 · kg-1, calculated according to the formula (A.3)
αm (20 ° C, D) = α lρα
(A.3)
Where.
α - the measured angle of rotation, in degrees (°);
l - the length of the rotating tube in dm (dm);
ρα - Mass concentration of the effective components in solution, in grams per milliliter (g/mL).
A.10.2 Others according to GB/T 613.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 25543-2010_English be delivered?Answer: Upon your order, we will start to translate GB 25543-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB 25543-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB 25543-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|